Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3401043..3401663 Replicon chromosome
Accession NZ_CP069159
Organism Citrobacter sp. R56

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag JM656_RS16080 Protein ID WP_002892050.1
Coordinates 3401445..3401663 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A8AJY4
Locus tag JM656_RS16075 Protein ID WP_012133514.1
Coordinates 3401043..3401417 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JM656_RS16065 3396176..3397369 + 1194 WP_203358643.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
JM656_RS16070 3397392..3400541 + 3150 WP_203358644.1 multidrug efflux RND transporter permease subunit AcrB -
JM656_RS16075 3401043..3401417 + 375 WP_012133514.1 Hha toxicity modulator TomB Antitoxin
JM656_RS16080 3401445..3401663 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
JM656_RS16085 3401847..3402398 + 552 WP_203358645.1 maltose O-acetyltransferase -
JM656_RS16090 3402513..3402983 + 471 WP_203358646.1 YlaC family protein -
JM656_RS16095 3403056..3403196 - 141 WP_192611404.1 type B 50S ribosomal protein L36 -
JM656_RS16100 3403198..3403458 - 261 WP_203358647.1 type B 50S ribosomal protein L31 -
JM656_RS16105 3403672..3405222 + 1551 WP_203358648.1 EAL domain-containing protein -
JM656_RS16110 3405262..3405615 - 354 WP_192611401.1 DUF1428 family protein -
JM656_RS16115 3405681..3406310 - 630 WP_203358649.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T190004 WP_002892050.1 NZ_CP069159:3401445-3401663 [Citrobacter sp. R56]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T190004 NZ_CP091058:c5442093-5441710 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTTGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGGTTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14437.27 Da        Isoelectric Point: 5.1444

>AT190004 WP_012133514.1 NZ_CP069159:3401043-3401417 [Citrobacter sp. R56]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT190004 NZ_CP091058:c5442335-5442093 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A8AJY4

References