Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3033775..3034429 Replicon chromosome
Accession NZ_CP069133
Organism Escherichia coli BW25113 substr. CHXR2G20

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag JQK31_RS14720 Protein ID WP_000244777.1
Coordinates 3033775..3034182 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag JQK31_RS14725 Protein ID WP_000354046.1
Coordinates 3034163..3034429 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JQK31_RS14700 3029732..3031465 - 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -
JQK31_RS14705 3031471..3032181 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
JQK31_RS14710 3032206..3033102 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
JQK31_RS14715 3033214..3033735 + 522 WP_001055874.1 flavodoxin FldB -
JQK31_RS14720 3033775..3034182 - 408 WP_000244777.1 toxin CptA Toxin
JQK31_RS14725 3034163..3034429 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
JQK31_RS14730 3034672..3035652 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
JQK31_RS14735 3035848..3036507 - 660 WP_000250274.1 hemolysin III family protein -
JQK31_RS14740 3036671..3036982 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
JQK31_RS14745 3037027..3038460 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
JQK31_RS14750 3038517..3039260 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T189976 WP_000244777.1 NZ_CP069133:c3034182-3033775 [Escherichia coli BW25113]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T189976 NZ_CP091044:c4442512-4442135 [Escherichia coli]
ATGAAAACATTACCTGATACACACGTACGGAAGGTATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGTACCGACCTGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGATCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT189976 WP_000354046.1 NZ_CP069133:c3034429-3034163 [Escherichia coli BW25113]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT189976 NZ_CP091044:c4442969-4442601 [Escherichia coli]
GTGTCAGACACACTCTCCGGGACAACGCATCCCGATGACAATCACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCACCCGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAATTAATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGTTACGTTTATCTGGCTTTTTATCCAACATCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References