Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2070589..2071420 Replicon chromosome
Accession NZ_CP069132
Organism Escherichia coli BW25113 substr. CHXR1G20

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag JQK35_RS10230 Protein ID WP_000854814.1
Coordinates 2071046..2071420 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID P76364
Locus tag JQK35_RS10225 Protein ID WP_001285584.1
Coordinates 2070589..2070957 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JQK35_RS10210 2068256..2069788 + 1533 WP_001350525.1 hypothetical protein -
JQK35_RS10215 2069785..2070231 + 447 WP_000187523.1 RadC family protein -
JQK35_RS10220 2070294..2070515 + 222 WP_000692323.1 DUF987 domain-containing protein -
JQK35_RS10225 2070589..2070957 + 369 WP_001285584.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
JQK35_RS10230 2071046..2071420 + 375 WP_000854814.1 type IV toxin-antitoxin system toxin CbtA Toxin
JQK35_RS10235 2071417..2071611 + 195 WP_000988600.1 hypothetical protein -
JQK35_RS10240 2071624..2071737 + 114 WP_001161660.1 DUF957 domain-containing protein -
JQK35_RS10245 2072026..2072154 - 129 Protein_2001 transposase domain-containing protein -
JQK35_RS10250 2072226..2072408 + 183 WP_001016348.1 hypothetical protein -
JQK35_RS10255 2072509..2072838 - 330 WP_000450409.1 DUF496 family protein -
JQK35_RS10260 2073010..2074068 - 1059 WP_001200891.1 FUSC family protein -
JQK35_RS10265 2074266..2074739 - 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
JQK35_RS10270 2074858..2076024 - 1167 WP_000830156.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T189928 WP_000854814.1 NZ_CP069132:2071046-2071420 [Escherichia coli BW25113]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T189928 NZ_CP091027:c84895-84617 [Escherichia coli]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAAGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGATGGC
CGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAACCAGTGGGGAAAAGTGTAT
ATCCTGCGTGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13651.52 Da        Isoelectric Point: 5.9541

>AT189928 WP_001285584.1 NZ_CP069132:2070589-2070957 [Escherichia coli BW25113]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT189928 NZ_CP091027:c85164-84895 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
PDB 2H28
AlphaFold DB A0A1M2E8G6

References