Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1503539..1504177 Replicon chromosome
Accession NZ_CP069132
Organism Escherichia coli BW25113 substr. CHXR1G20

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag JQK35_RS07350 Protein ID WP_000813794.1
Coordinates 1503539..1503715 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag JQK35_RS07355 Protein ID WP_001270286.1
Coordinates 1503761..1504177 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JQK35_RS07330 1499158..1500372 - 1215 WP_001326689.1 BenE family transporter YdcO -
JQK35_RS07335 1500425..1500961 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
JQK35_RS07340 1501034..1502995 + 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
JQK35_RS07345 1503087..1503317 - 231 WP_000494244.1 YncJ family protein -
JQK35_RS07350 1503539..1503715 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
JQK35_RS07355 1503761..1504177 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
JQK35_RS07360 1504256..1505662 + 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
JQK35_RS07365 1505907..1507052 + 1146 WP_000047424.1 ABC transporter substrate-binding protein -
JQK35_RS07370 1507070..1508083 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
JQK35_RS07375 1508084..1509025 + 942 WP_001251304.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T189917 WP_000813794.1 NZ_CP069132:1503539-1503715 [Escherichia coli BW25113]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T189917 NZ_CP091026:c1957558-1957184 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT189917 WP_001270286.1 NZ_CP069132:1503761-1504177 [Escherichia coli BW25113]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT189917 NZ_CP091026:c1957952-1957647 [Escherichia coli]
CTGCCCTGTACCGTGACGCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGC
CGGTATCAGAGGCCTGTTCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAAC
TCATGCTCACCAGCGGTGAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCC
GATACCCTCAGCAGTTGTGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References