Detailed information of TA system TA189905

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 259035..259714
• Replicon: chromosome
• Accession: NZ_CP069132
• Organism: Escherichia coli BW25113 substr. CHXR1G20

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: P77692
• Locus tag: JQK35_RS01230
• Protein ID: WP_000854672.1
• Coordinates: 259035..259376 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: Q47684
• Locus tag: JQK35_RS01235
• Protein ID: WP_000070395.1
• Coordinates: 259397..259714 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JQK35_RS01205	254312..254713	+	402	WP_000174689.1	sigma factor-binding protein Crl	-
JQK35_RS01210	254752..255807	-	1056	WP_000749863.1	phosphoporin PhoE	-
JQK35_RS01215	256095..257198	+	1104	WP_001285288.1	glutamate 5-kinase	-
JQK35_RS01220	257210..258463	+	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
JQK35_RS01230	259035..259376	-	342	WP_000854672.1	type IV toxin-antitoxin system toxin YkfI	Toxin
JQK35_RS01235	259397..259714	-	318	WP_000070395.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
JQK35_RS01240	259733..259954	-	222	WP_000691994.1	DUF987 domain-containing protein	-
JQK35_RS01245	259963..260439	-	477	WP_000811693.1	RadC family protein	-
JQK35_RS01250	260455..260913	-	459	WP_000211838.1	antirestriction protein	-
JQK35_RS01255	261011..261250	-	240	WP_000194654.1	DUF905 domain-containing protein	-
JQK35_RS01260	261327..261794	-	468	WP_001547765.1	hypothetical protein	-
JQK35_RS01265	261817..262260	-	444	WP_000824223.1	hypothetical protein	-
JQK35_RS01270	262260..262487	-	228	WP_001548158.1	hypothetical protein	-
JQK35_RS01275	262483..262674	-	192	Protein_246	DeoR family transcriptional regulator	-
JQK35_RS01280	262891..263712	-	822	WP_000197389.1	DUF945 domain-containing protein	-
JQK35_RS01285	263804..264667	-	864	WP_001065553.1	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.01 Da
• Isoelectric Point: 9.6543

>T189905 WP_000854672.1 NZ_CP069132:c259376-259035 [Escherichia coli BW25113]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T189905 NZ_CP091025:c29411-29013 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTTACGATAAAGAACAAACCCGCCAGTGTCAGGGAACGTTTTAA
CCTGAACCAGGGGAGAATGTGCATCAGTTCGGTCACTCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGACGTTCTGGATTACGACGCTGCTGCTGCCACACACACC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGGCGGCCTGAGAATTGAAGACTGGAGTTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11939.59 Da
• Isoelectric Point: 6.7391

>AT189905 WP_000070395.1 NZ_CP069132:c259714-259397 [Escherichia coli BW25113]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT189905 NZ_CP091025:c29638-29411 [Escherichia coli]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCCGAATCATTACGCCTGCCGGAGAGACATGGGACGAATGGTTCGACGGAA
ACAGTGTCAGCGCTGATTTTATGGATAACAGGGAACAGCCTGGTATGCAGGAAAGGGAGTCATTCTGA

Structures

Toxin

• AlphaFold DB: P77692

Antitoxin

• AlphaFold DB: Q47684