Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 522875..523528 Replicon chromosome
Accession NZ_CP069127
Organism Brevibacillus choshinensis strain HPD31-SP3

Toxin (Protein)


Gene name mazF Uniprot ID J2PLP3
Locus tag JNE38_RS02840 Protein ID WP_005830205.1
Coordinates 523178..523528 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A3M8AYL7
Locus tag JNE38_RS02835 Protein ID WP_025845698.1
Coordinates 522875..523174 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNE38_RS02820 519162..520940 + 1779 WP_203355166.1 adenine deaminase -
JNE38_RS02825 521005..521445 + 441 WP_203355167.1 hypothetical protein -
JNE38_RS02830 521448..522653 + 1206 WP_203355168.1 alanine racemase -
JNE38_RS02835 522875..523174 + 300 WP_025845698.1 CopG family ribbon-helix-helix protein Antitoxin
JNE38_RS02840 523178..523528 + 351 WP_005830205.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
JNE38_RS02845 523808..524206 + 399 WP_203357394.1 anti-sigma regulatory factor -
JNE38_RS02850 524227..525228 + 1002 WP_203355169.1 PP2C family protein-serine/threonine phosphatase -
JNE38_RS02855 525252..525578 + 327 WP_203355170.1 anti-sigma factor antagonist -
JNE38_RS02860 525578..526072 + 495 WP_203355171.1 anti-sigma B factor RsbW -
JNE38_RS02865 526041..526829 + 789 WP_203355172.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(15-52)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12917.86 Da        Isoelectric Point: 4.8435

>T189902 WP_005830205.1 NZ_CP069127:523178-523528 [Brevibacillus choshinensis]
VIVKRGDVFFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKTYGFDRDSVILLEQI
RTIDKQRLTDKITHLDDEMMDRVNESLQISLGLIDF

Download         Length: 351 bp

>T189902 NZ_CP091024:c4460879-4460529 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 11323.26 Da        Isoelectric Point: 10.3594

>AT189902 WP_025845698.1 NZ_CP069127:522875-523174 [Brevibacillus choshinensis]
MVGGGFVLSKSNTKRIMISLPQHLLQEVDGVIQKEKSNRSEFIRQAMHLYLKERKKRTIRETMQKGYMEMAKINLKMASE
AFGAEEEADHTLVRLVSGV

Download         Length: 300 bp

>AT189902 NZ_CP091024:c4461124-4460873 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCTTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB J2PLP3

Antitoxin

Source ID Structure
AlphaFold DB A0A3M8AYL7

References