Detailed information of TA system TA189892

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-dinJ/YafQ-RelB
• Location: 84318..84849
• Replicon: plasmid pLWPZF
• Accession: NZ_CP069081
• Organism: Pseudomonas putida strain LWPZF

Toxin (Protein)

• Gene name: relE
• Uniprot ID: R4IU69
• Locus tag: JNO42_RS29320
• Protein ID: WP_016355700.1
• Coordinates: 84568..84849 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: R4ITX8
• Locus tag: JNO42_RS29315
• Protein ID: WP_016355699.1
• Coordinates: 84318..84581 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNO42_RS29285 (JNO42_29115)	80153..80365	+	213	WP_134939350.1	hypothetical protein	-
JNO42_RS29290	80435..80631	+	197	Protein_87	YeeE/YedE family protein	-
JNO42_RS29850 (JNO42_29120)	80662..83518	-	2857	Protein_88	Tn3 family transposase	-
JNO42_RS29310 (JNO42_29125)	83545..84108	-	564	WP_172773422.1	recombinase family protein	-
JNO42_RS29315 (JNO42_29130)	84318..84581	+	264	WP_016355699.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
JNO42_RS29320 (JNO42_29135)	84568..84849	+	282	WP_016355700.1	type II toxin-antitoxin system YafQ family toxin	Toxin
JNO42_RS29325 (JNO42_29140)	85060..85332	+	273	Protein_92	SDR family NAD(P)-dependent oxidoreductase	-
JNO42_RS29330 (JNO42_29145)	85387..86413	+	1027	Protein_93	IS30 family transposase	-
JNO42_RS29335 (JNO42_29150)	86445..86984	+	540	Protein_94	group II intron maturase-specific domain-containing protein	-
JNO42_RS29340 (JNO42_29155)	87065..87442	+	378	Protein_95	IS3 family transposase	-
JNO42_RS29345 (JNO42_29160)	87576..88259	+	684	WP_009683812.1	serine acetyltransferase	-
JNO42_RS29350 (JNO42_29165)	88265..89608	-	1344	WP_009683813.1	aminodeoxychorismate synthase component I	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..160969	160969
	-	inside	IScluster/Tn	-	-	76914..87505	10591

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (1-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-84)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10742.21 Da
• Isoelectric Point: 9.0304

>T189892 WP_016355700.1 NZ_CP069081:84568-84849 [Pseudomonas putida]
MRTFEQTSRFKRDYKREAKGPHRQTLASDFVAVVTALANDRVLAEKHHDHALTGDWKDHRDCHIKPDLVLIYRKPDDAVL
QLVRLGSHSELGL

Nucleotide

• Length: 282 bp

>T189892 NZ_CP091024:1940345-1940596 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9485.04 Da
• Isoelectric Point: 5.8317

>AT189892 WP_016355699.1 NZ_CP069081:84318-84581 [Pseudomonas putida]
MSANAVVRARIDEHIKEEATVVLAAMGLTVSDAFRIMLTRVAREKALPFEPLVPNTTTIEAMKEARRGGLKSFATVEDLM
ADLNANV

Nucleotide

• Length: 264 bp

>AT189892 NZ_CP091024:1940593-1940847 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: R4IU69

Antitoxin

• AlphaFold DB: R4ITX8