Detailed information of TA system TA189891

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 5679528..5680114
• Replicon: chromosome
• Accession: NZ_CP069080
• Organism: Pseudomonas putida strain LWPZF

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: JNO42_RS26095
• Protein ID: WP_239608161.1
• Coordinates: 5679528..5679821 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: I3UY28
• Locus tag: JNO42_RS26100
• Protein ID: WP_004576347.1
• Coordinates: 5679818..5680114 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNO42_RS26070 (JNO42_25910)	5675129..5675983	+	855	WP_239607061.1	putative selenate ABC transporter substrate-binding protein	-
JNO42_RS26075 (JNO42_25915)	5675980..5676789	+	810	WP_239607062.1	phosphonate ABC transporter ATP-binding protein	-
JNO42_RS26080 (JNO42_25920)	5676783..5677610	+	828	WP_004576350.1	ABC transporter permease subunit	-
JNO42_RS26085 (JNO42_25925)	5677607..5678374	+	768	WP_121782880.1	phosphonate ABC transporter, permease protein PhnE	-
JNO42_RS26090 (JNO42_25930)	5678411..5679304	-	894	WP_239607063.1	bestrophin family ion channel	-
JNO42_RS26095 (JNO42_25935)	5679528..5679821	+	294	WP_239608161.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JNO42_RS26100 (JNO42_25940)	5679818..5680114	+	297	WP_004576347.1	putative addiction module antidote protein	Antitoxin
JNO42_RS26105 (JNO42_25945)	5680125..5680604	-	480	WP_014861464.1	GNAT family N-acetyltransferase	-
JNO42_RS26110 (JNO42_25950)	5680775..5681422	-	648	WP_239608163.1	serine protease	-
JNO42_RS26115 (JNO42_25955)	5681766..5682188	+	423	WP_239608165.1	VOC family protein	-
JNO42_RS26120 (JNO42_25960)	5682355..5682948	+	594	WP_239607064.1	hypothetical protein	-
JNO42_RS26125 (JNO42_25965)	5683157..5683981	-	825	WP_239607065.1	DUF4393 domain-containing protein	-
JNO42_RS26130 (JNO42_25970)	5684526..5684885	-	360	WP_239607066.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (4-96)

Antitoxin

• Uncharacterized protein HI1420 (4-93)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10735.51 Da
• Isoelectric Point: 10.8302

>T189891 WP_239608161.1 NZ_CP069080:5679528-5679821 [Pseudomonas putida]
MFLVEQTESFAAWLSSLRDLRARLAIGRRLERAAAGNLGDFKWPGGGIGELRIDVAAGYRVYFTQKGQRLVLLLVGGDKS
TQAADILNARKLLKEMK

Nucleotide

• Length: 294 bp

>T189891 NZ_CP091024:1362331-1362729 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACT
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10676.33 Da
• Isoelectric Point: 6.7249

>AT189891 WP_004576347.1 NZ_CP069080:5679818-5680114 [Pseudomonas putida]
MSEKLIPFDMAALLSSDEAISEYLSQVLSNGDTEEIIRALGHIARARGMTHIATESGLGRESLYKALSPGAKPRFDTVLR
VIRALGVDLLVQPHAMTR

Nucleotide

• Length: 297 bp

>AT189891 NZ_CP091024:1362104-1362331 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A2I0CD26