Detailed information of TA system TA189890

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 4411601..4413330
• Replicon: chromosome
• Accession: NZ_CP069080
• Organism: Pseudomonas putida strain LWPZF

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: JNO42_RS20355
• Protein ID: WP_019752695.1
• Coordinates: 4412689..4413330 (-)
• Length: 214 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: I3UUH2
• Locus tag: JNO42_RS20350
• Protein ID: WP_014754747.1
• Coordinates: 4411601..4412689 (-)
• Length: 363 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNO42_RS20335 (JNO42_20210)	4407231..4408049	-	819	WP_173881454.1	HDOD domain-containing protein	-
JNO42_RS20340 (JNO42_20215)	4408225..4409421	+	1197	WP_004575470.1	class I SAM-dependent rRNA methyltransferase	-
JNO42_RS20345 (JNO42_20220)	4409692..4411533	+	1842	WP_239606901.1	dihydroxy-acid dehydratase	-
JNO42_RS20350 (JNO42_20225)	4411601..4412689	-	1089	WP_014754747.1	macro domain-containing protein	Antitoxin
JNO42_RS20355 (JNO42_20230)	4412689..4413330	-	642	WP_019752695.1	DUF4433 domain-containing protein	Toxin
JNO42_RS20360 (JNO42_20235)	4413327..4413905	-	579	WP_019752696.1	DarT ssDNA thymidine ADP-ribosyltransferase family protein	-
JNO42_RS20365 (JNO42_20240)	4414003..4415058	-	1056	WP_239606902.1	haloacid dehalogenase-like hydrolase	-
JNO42_RS20370 (JNO42_20245)	4415303..4416694	+	1392	WP_239606903.1	L-cystine transporter	-
JNO42_RS20375 (JNO42_20250)	4416971..4417486	-	516	WP_239606904.1	dihydrofolate reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (14-213)

Antitoxin

• Macro domain (18-130)

Sequences

Toxin

Protein

• Length: 214 a.a.
• Molecular weight: 24591.17 Da
• Isoelectric Point: 9.3541

>T189890 WP_019752695.1 NZ_CP069080:c4413330-4412689 [Pseudomonas putida]
MIYSSLNPSKALIWRIVHRDNLSWVLDHGLHAVSSGKLSSTYVGIGNPELISKRAQRRVPLEPNGTLADYVPFYFTPFSV
MMKNIHSGWGVPQRRNEEIVILVSSLYHAQSLGLQFVFTDAHAYPHWAGYYSDLTQLDKIDWEILQRRDFKRDPDDPRKM
ERYQAEALIHGHVPVAALLGIVCYNEALKGRVDKEVQQRRLNLPVYARPGWYF

Nucleotide

• Length: 642 bp

>T189890 NZ_CP091024:1070618-1070953 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 363 a.a.
• Molecular weight: 40625.75 Da
• Isoelectric Point: 6.5916

>AT189890 WP_014754747.1 NZ_CP069080:c4412689-4411601 [Pseudomonas putida]
MIHFMQGNLLEAKAEALVNTVNTVGVMGKGIALMFKERFVENYRQYAAACKAKEVVTGKMFVTEVRELDGPRWIVNFPTK
RHWRSPSQIGWIVEGLHDLRNFIINNQVASIAIPPLGAGNGGLSWAAVREQIQRVLGDLDADVFVFEPTANYQNVSKRSG
VEVLTPARALIAELVRRYWVLGMECSLLEVQKLAWFLERSIERFTPGINPLELKFVAHKYGPYANRLEHLLDNLDGSYLH
CRKRISDAGPLDVIWFDEGRKSFLQAYLNSEAKAYLPALEFTAELIDGFESPFGMELLATVDWLLHEDGVEPTVPAVREH
LKNWSGGVGAAARKSKLFDDEALGIALQKLSMFRSDPATSLH

Nucleotide

• Length: 1089 bp

>AT189890 NZ_CP091024:1070370-1070618 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Antitoxin

• AlphaFold DB: I3UUH2