Detailed information of TA system TA189886

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 533723..534762
• Replicon: chromosome
• Accession: NZ_CP069080
• Organism: Pseudomonas putida strain LWPZF

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: JNO42_RS02320
• Protein ID: WP_019750859.1
• Coordinates: 533723..534298 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: JNO42_RS02325
• Protein ID: WP_239607231.1
• Coordinates: 534295..534762 (-)
• Length: 156 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNO42_RS02310 (JNO42_02305)	529340..530575	+	1236	WP_239607229.1	FAD-dependent oxidoreductase	-
JNO42_RS02315 (JNO42_02310)	530579..533509	-	2931	WP_239607230.1	aminotransferase	-
JNO42_RS02320 (JNO42_02315)	533723..534298	-	576	WP_019750859.1	PIN domain-containing protein	Toxin
JNO42_RS02325 (JNO42_02320)	534295..534762	-	468	WP_239607231.1	helix-turn-helix domain-containing protein	Antitoxin
JNO42_RS02330 (JNO42_02325)	534907..536514	-	1608	WP_143493761.1	ABC transporter ATP-binding protein	-
JNO42_RS02335 (JNO42_02330)	536516..537535	-	1020	WP_003251467.1	ABC transporter permease	-
JNO42_RS02340 (JNO42_02335)	537537..538610	-	1074	WP_003251466.1	microcin C ABC transporter permease YejB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-115)

Antitoxin

• Helix-turn-helix domain, group 17 (84-132)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21683.92 Da
• Isoelectric Point: 5.5710

>T189886 WP_019750859.1 NZ_CP069080:c534298-533723 [Pseudomonas putida]
MKHSPFTAIYDANVLYPAPLRDFLMNLALTGIYRARWSASIHDEWKRNLLLNRPDLTPEHLDRTSSLMDAAVPDALVTDY
DSLVEGLDLPDRDDRHVLAAAIKCNASVIVTFNLKDFPKAVLGAFDIEPLHPDDFIADLWDLDKAAVLEAAQRQRISLKN
PPHNVQQYLDRLLQQKLPETVKLLSAFKFLL

Nucleotide

• Length: 576 bp

>T189886 NZ_CP091024:324028-324555 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATAAAAAAGCCCATACGTTTTATCAATCGCTGGGCTTTATCCCGTTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATCGAACTGCTTTTCACACAGAGCGATTAA

Antitoxin

Protein

• Length: 156 a.a.
• Molecular weight: 17243.74 Da
• Isoelectric Point: 6.6505

>AT189886 WP_239607231.1 NZ_CP069080:c534762-534295 [Pseudomonas putida]
MTTANLSRHVLPDEKEIAVAVESSRTLAAFLSTKSDTQRIELLNEENQRQVVEVPTFALRLFGEILSELALGNTVKVVPI
HAELTTQEAADLLNVSRPHLVKLLDEAVIPHTKTGRHRRVKFSDLVAYKDKRDGVSRTAMDALAAQAQELKMGYE

Nucleotide

• Length: 468 bp

>AT189886 NZ_CP091024:323755-324021 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCTGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA