Detailed information of TA system TA189881

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/Txe-YefM
• Location: 3767430..3767959
• Replicon: chromosome
• Accession: NZ_CP069078
• Organism: Mycobacterium tuberculosis strain N0157

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G0TI95
• Locus tag: IMW72_RS17745
• Protein ID: WP_003417760.1
• Coordinates: 3767702..3767959 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G0TI94
• Locus tag: IMW72_RS17740
• Protein ID: WP_003417757.1
• Coordinates: 3767430..3767705 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMW72_RS17705	3764003..3764692	-	690	WP_003917774.1	BBE domain-containing protein	-
IMW72_RS17710	3764879..3765250	-	372	WP_003417739.1	FAD-binding protein	-
IMW72_RS17715	3765148..3765366	-	219	WP_157132559.1	hypothetical protein	-
IMW72_RS17720	3765393..3765653	-	261	WP_003417741.1	hypothetical protein	-
IMW72_RS17725	3765768..3766157	+	390	WP_003417745.1	DUF732 domain-containing protein	-
IMW72_RS17730	3766171..3766464	-	294	WP_003417749.1	DUF3017 domain-containing protein	-
IMW72_RS17735	3766461..3767306	-	846	WP_003905054.1	bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase	-
IMW72_RS17740	3767430..3767705	+	276	WP_003417757.1	type II toxin-antitoxin system antitoxin RelJ	Antitoxin
IMW72_RS17745	3767702..3767959	+	258	WP_003417760.1	Txe/YoeB family addiction module toxin	Toxin
IMW72_RS17750	3768001..3769191	+	1191	WP_003900033.1	NADH:flavin oxidoreductase	-
IMW72_RS17755	3769308..3769676	+	369	WP_003417765.1	FHA domain-containing protein	-
IMW72_RS17760	3769673..3770224	-	552	WP_003417767.1	pentapeptide repeat protein MfpA	-
IMW72_RS17765	3770231..3770812	-	582	WP_003417769.1	ATP/GTP-binding protein	-
IMW72_RS17770	3770793..3771161	-	369	WP_003417772.1	DUF742 domain-containing protein	-
IMW72_RS17775	3771139..3771531	-	393	WP_003417776.1	serine protease inhibitor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (6-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-72)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10070.30 Da
• Isoelectric Point: 6.4693

>T189881 WP_003417760.1 NZ_CP069078:3767702-3767959 [Mycobacterium tuberculosis]
VRSVNFDPDAWEDFLFWLAADRKTARRITRLIGEIQRDPFSGIGKPEPLQGELSGYWSRRIDDEHRLVYRAGDDEVTMLK
ARYHY

Nucleotide

• Length: 258 bp

>T189881 NZ_CP091023:c60771-60496 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCACTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTGCAGTCCCTGACACAGGCGCCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTTCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10194.35 Da
• Isoelectric Point: 4.6652

>AT189881 WP_003417757.1 NZ_CP069078:3767430-3767705 [Mycobacterium tuberculosis]
MSISASEARQRLFPLIEQVNTDHQPVRITSRAGDAVLMSADDYDAWQETVYLLRSPENARRLMEAVARDKAGHSAFTKSV
DELREMAGGEE

Nucleotide

• Length: 276 bp

>AT189881 NZ_CP091023:c61055-60771 [Escherichia coli]
ATGCAAATGAAAAACAATAGTGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGGCTGGAACGATCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGACCTGG
GCAGACAGCCTCAATACTGACAATCCGTTACCGGTGCCTCGCTGA

Structures

Toxin

• PDB: 3OEI

Antitoxin

• PDB: 3CTO
• PDB: 3OEI
• PDB: 3D55
• AlphaFold DB: G0TI94