Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 3177750..3178437 Replicon chromosome
Accession NZ_CP069078
Organism Mycobacterium tuberculosis strain N0157

Toxin (Protein)


Gene name vapC Uniprot ID P65044
Locus tag IMW72_RS15165 Protein ID WP_003414624.1
Coordinates 3177994..3178437 (+) Length 148 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TFH0
Locus tag IMW72_RS15160 Protein ID WP_003414620.1
Coordinates 3177750..3178007 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMW72_RS15140 3173070..3173924 - 855 WP_003899516.1 GNAT family N-acetyltransferase -
IMW72_RS15145 3173980..3175143 - 1164 WP_003899517.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
IMW72_RS15150 3175160..3176374 - 1215 WP_003414610.1 zinc metalloprotease Rip -
IMW72_RS15155 3176382..3177623 - 1242 WP_003414613.1 1-deoxy-D-xylulose-5-phosphate reductoisomerase -
IMW72_RS15160 3177750..3178007 + 258 WP_003414620.1 ribbon-helix-helix domain-containing protein Antitoxin
IMW72_RS15165 3177994..3178437 + 444 WP_003414624.1 type II toxin-antitoxin system VapC family toxin Toxin
IMW72_RS15170 3178517..3179179 + 663 WP_003414630.1 cell surface glycolipoprotein Mpt83 -
IMW72_RS15175 3179275..3179466 + 192 WP_003414632.1 type II toxin-antitoxin system prevent-host-death family antitoxin -
IMW72_RS15180 3179798..3181546 + 1749 WP_003912869.1 cytochrome c biogenesis protein DipZ -
IMW72_RS15185 3181642..3182223 + 582 WP_003414644.1 fasciclin domain-containing protein -
IMW72_RS15190 3182323..3182589 + 267 WP_015456449.1 DUF2631 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain toxin

(1-142)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 148 a.a.        Molecular weight: 16595.72 Da        Isoelectric Point: 6.4890

>T189878 WP_003414624.1 NZ_CP069078:3177994-3178437 [Mycobacterium tuberculosis]
MLCVDVNVLVYAHRADLREHADYRGLLERLANDDEPLGLPDSVLAGFIRVVTNRRVFTEPTSPQDAWQAVDALLAAPAAM
RLRPGERHWMAFRQLASDVDANGNDIADAHLAAYALENNATWLSADRGFARFRRLRWRHPLDGQTHL

Download         Length: 444 bp

>T189878 NZ_CP091022:c4391188-4391015 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9013.22 Da        Isoelectric Point: 10.8344

>AT189878 WP_003414620.1 NZ_CP069078:3177750-3178007 [Mycobacterium tuberculosis]
VRTTIRIDDELYREVKAKAARSGRTVAAVLEDAVRRGLNPPKPQAAGRYRVQPSGKGGLRPGVDLSSNAALAEAMNDGVS
VDAVR

Download         Length: 258 bp

>AT189878 NZ_CP091022:c4391512-4391216 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7VWO

Antitoxin

Source ID Structure
PDB 7VWO
AlphaFold DB G0TFH0

References