Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN_Sll0205-like-Phd
Location 3131232..3131836 Replicon chromosome
Accession NZ_CP069078
Organism Mycobacterium tuberculosis strain N0157

Toxin (Protein)


Gene name vapC Uniprot ID P71623
Locus tag IMW72_RS14940 Protein ID WP_003414492.1
Coordinates 3131232..3131624 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A829CBY8
Locus tag IMW72_RS14945 Protein ID WP_003414495.1
Coordinates 3131621..3131836 (-) Length 72 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMW72_RS14910 3126382..3127170 - 789 WP_003917092.1 CRISPR-associated endoribonuclease Cas6 -
IMW72_RS14915 3127504..3128049 - 546 WP_003904931.1 DUF1802 family protein -
IMW72_RS14920 3128321..3129205 - 885 WP_003414409.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
IMW72_RS14925 3129208..3130095 - 888 WP_003414414.1 type IV toxin-antitoxin system AbiEi family antitoxin -
IMW72_RS14930 3130400..3130945 - 546 WP_003908074.1 DUF1802 family protein -
IMW72_RS14935 3130942..3131211 - 270 WP_003414489.1 DUF2277 family protein -
IMW72_RS14940 3131232..3131624 - 393 WP_003414492.1 type II toxin-antitoxin system VapC family toxin Toxin
IMW72_RS14945 3131621..3131836 - 216 WP_003414495.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
IMW72_RS14950 3131883..3132632 + 750 WP_003908075.1 enoyl-CoA hydratase -
IMW72_RS14955 3132711..3133793 - 1083 WP_003908076.1 ABC transporter ATP-binding protein -
IMW72_RS14960 3133786..3135096 - 1311 WP_003414501.1 ABC transporter substrate-binding protein -
IMW72_RS14965 3135099..3135926 - 828 WP_003904072.1 carbohydrate ABC transporter permease -
IMW72_RS14970 3135923..3136834 - 912 WP_003904073.1 sugar ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

VapC-like Sll0205 protein, PIN domain

(5-130)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(2-46)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14610.74 Da        Isoelectric Point: 6.7594

>T189876 WP_003414492.1 NZ_CP069078:c3131624-3131232 [Mycobacterium tuberculosis]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Download         Length: 393 bp

>T189876 NZ_CP091022:c3789261-3788863 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 72 a.a.        Molecular weight: 7511.55 Da        Isoelectric Point: 4.9732

>AT189876 WP_003414495.1 NZ_CP069078:c3131836-3131621 [Mycobacterium tuberculosis]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Download         Length: 216 bp

>AT189876 NZ_CP091022:c3789557-3789264 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BWM8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CBY8

References