Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3103497..3104067 Replicon chromosome
Accession NZ_CP069078
Organism Mycobacterium tuberculosis strain N0157

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag IMW72_RS14795 Protein ID WP_003414166.1
Coordinates 3103497..3103853 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag IMW72_RS14800 Protein ID WP_003901465.1
Coordinates 3103837..3104067 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMW72_RS14775 3098949..3100637 - 1689 WP_003414155.1 alpha/beta hydrolase family protein -
IMW72_RS14780 3100641..3100967 - 327 WP_003414157.1 hypothetical protein -
IMW72_RS14785 3101140..3101727 + 588 WP_003914429.1 DUF3558 family protein -
IMW72_RS14790 3101746..3103395 + 1650 WP_003899485.1 CocE/NonD family hydrolase -
IMW72_RS14795 3103497..3103853 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
IMW72_RS14800 3103837..3104067 - 231 WP_003901465.1 type II toxin-antitoxin system antitoxin MazE9 Antitoxin
IMW72_RS14805 3104110..3105153 - 1044 WP_003414172.1 DUF2293 domain-containing protein -
IMW72_RS14810 3105152..3105619 + 468 WP_003414177.1 DUF1778 domain-containing protein -
IMW72_RS14815 3105795..3106049 - 255 WP_003917684.1 hypothetical protein -
IMW72_RS14820 3106197..3106601 + 405 WP_003414181.1 hypothetical protein -
IMW72_RS14825 3106598..3106789 + 192 WP_003414184.1 hypothetical protein -
IMW72_RS14830 3107006..3107266 + 261 Protein_2927 transposase -
IMW72_RS14835 3108376..3108633 + 258 WP_003899489.1 hypothetical protein -
IMW72_RS14840 3108738..3109049 + 312 WP_003414190.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T189874 WP_003414166.1 NZ_CP069078:c3103853-3103497 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T189874 NZ_CP091022:2819161-2819268 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTACTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT189874 WP_003901465.1 NZ_CP069078:c3104067-3103837 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT189874 NZ_CP091022:c2819111-2819048 [Escherichia coli]
TCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References