Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 3063912..3064599 Replicon chromosome
Accession NZ_CP069078
Organism Mycobacterium tuberculosis strain N0157

Toxin (Protein)


Gene name vapC Uniprot ID G0TF65
Locus tag IMW72_RS14575 Protein ID WP_003414064.1
Coordinates 3064204..3064599 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TF64
Locus tag IMW72_RS14570 Protein ID WP_003414061.1
Coordinates 3063912..3064178 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMW72_RS14540 3059551..3060453 - 903 WP_003900564.1 4-hydroxy-tetrahydrodipicolinate synthase -
IMW72_RS14545 3060522..3061274 - 753 WP_003899465.1 FAD-dependent thymidylate synthase -
IMW72_RS14555 3061518..3061793 - 276 WP_003414055.1 type I restriction endonuclease subunit S -
IMW72_RS14560 3061790..3063412 - 1623 WP_003414057.1 class I SAM-dependent DNA methyltransferase -
IMW72_RS14565 3063499..3063915 - 417 WP_003414059.1 type II toxin-antitoxin system toxin ribonuclease C21 -
IMW72_RS14570 3063912..3064178 - 267 WP_003414061.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
IMW72_RS14575 3064204..3064599 - 396 WP_003414064.1 type II toxin-antitoxin system VapC family toxin Toxin
IMW72_RS14580 3064596..3064865 - 270 WP_003414066.1 type II toxin-antitoxin system VapB family antitoxin -
IMW72_RS14585 3064875..3065969 - 1095 WP_003414068.1 restriction endonuclease subunit S -
IMW72_RS14590 3065966..3066385 - 420 WP_003414070.1 winged helix-turn-helix domain-containing protein -
IMW72_RS14595 3066384..3066458 + 75 Protein_2880 hypothetical protein -
IMW72_RS14600 3066459..3066938 - 480 WP_003414073.1 dihydrofolate reductase -
IMW72_RS14605 3067009..3067809 - 801 WP_003911953.1 thymidylate synthase -
IMW72_RS14610 3067965..3068702 + 738 WP_003414079.1 dienelactone hydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-125)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(16-56)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14340.04 Da        Isoelectric Point: 4.7145

>T189873 WP_003414064.1 NZ_CP069078:c3064599-3064204 [Mycobacterium tuberculosis]
VIVDTSAIVAIVSGESGAQVLKEALERSPNSRMSAPNYVELCAIMQRRDRPEISRLVDRLLDDYGIQVEAVDADQARVAA
QAYRDYGRGSGHPARLNLGDTYSYALAQVTGEPLLFRGDDFTHTDIRPACT

Download         Length: 396 bp

>T189873 NZ_CP091022:2817179-2817286 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9493.77 Da        Isoelectric Point: 7.2783

>AT189873 WP_003414061.1 NZ_CP069078:c3064178-3063912 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Download         Length: 267 bp

>AT189873 NZ_CP091022:c2817132-2817066 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TF65

Antitoxin

Source ID Structure
AlphaFold DB G0TF64

References