Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 3063499..3064178 Replicon chromosome
Accession NZ_CP069078
Organism Mycobacterium tuberculosis strain N0157

Toxin (Protein)


Gene name vapC Uniprot ID P9WF90
Locus tag IMW72_RS14565 Protein ID WP_003414059.1
Coordinates 3063499..3063915 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TF64
Locus tag IMW72_RS14570 Protein ID WP_003414061.1
Coordinates 3063912..3064178 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMW72_RS14540 3059551..3060453 - 903 WP_003900564.1 4-hydroxy-tetrahydrodipicolinate synthase -
IMW72_RS14545 3060522..3061274 - 753 WP_003899465.1 FAD-dependent thymidylate synthase -
IMW72_RS14555 3061518..3061793 - 276 WP_003414055.1 type I restriction endonuclease subunit S -
IMW72_RS14560 3061790..3063412 - 1623 WP_003414057.1 class I SAM-dependent DNA methyltransferase -
IMW72_RS14565 3063499..3063915 - 417 WP_003414059.1 type II toxin-antitoxin system toxin ribonuclease C21 Toxin
IMW72_RS14570 3063912..3064178 - 267 WP_003414061.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
IMW72_RS14575 3064204..3064599 - 396 WP_003414064.1 type II toxin-antitoxin system VapC family toxin -
IMW72_RS14580 3064596..3064865 - 270 WP_003414066.1 type II toxin-antitoxin system VapB family antitoxin -
IMW72_RS14585 3064875..3065969 - 1095 WP_003414068.1 restriction endonuclease subunit S -
IMW72_RS14590 3065966..3066385 - 420 WP_003414070.1 winged helix-turn-helix domain-containing protein -
IMW72_RS14595 3066384..3066458 + 75 Protein_2880 hypothetical protein -
IMW72_RS14600 3066459..3066938 - 480 WP_003414073.1 dihydrofolate reductase -
IMW72_RS14605 3067009..3067809 - 801 WP_003911953.1 thymidylate synthase -
IMW72_RS14610 3067965..3068702 + 738 WP_003414079.1 dienelactone hydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-123)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(16-56)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15773.00 Da        Isoelectric Point: 7.1294

>T189872 WP_003414059.1 NZ_CP069078:c3063915-3063499 [Mycobacterium tuberculosis]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Download         Length: 417 bp

>T189872 NZ_CP091022:2816644-2816751 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9493.77 Da        Isoelectric Point: 7.2783

>AT189872 WP_003414061.1 NZ_CP069078:c3064178-3063912 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Download         Length: 267 bp

>AT189872 NZ_CP091022:c2816597-2816531 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGGTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5SV2

Antitoxin

Source ID Structure
AlphaFold DB G0TF64

References