Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2925115..2925829 Replicon chromosome
Accession NZ_CP069078
Organism Mycobacterium tuberculosis strain N0157

Toxin (Protein)


Gene name vapC Uniprot ID G0TQK0
Locus tag IMW72_RS13740 Protein ID WP_003413460.1
Coordinates 2925389..2925829 (+) Length 147 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ20
Locus tag IMW72_RS13735 Protein ID WP_003413456.1
Coordinates 2925115..2925402 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMW72_RS13700 2920537..2920782 + 246 WP_003413429.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
IMW72_RS13705 2920779..2921183 + 405 WP_003413432.1 type II toxin-antitoxin system VapC family toxin -
IMW72_RS13710 2921400..2922020 + 621 WP_003413441.1 DUF4178 domain-containing protein -
IMW72_RS13715 2922031..2922525 + 495 WP_003413444.1 DUF2617 family protein -
IMW72_RS13720 2922522..2922953 + 432 WP_003413445.1 DUF4247 domain-containing protein -
IMW72_RS13725 2922978..2923436 + 459 WP_003413451.1 DUF350 domain-containing protein -
IMW72_RS13730 2923433..2925004 + 1572 WP_003899392.1 polyamine aminopropyltransferase -
IMW72_RS13735 2925115..2925402 + 288 WP_003413456.1 antitoxin VapB41 Antitoxin
IMW72_RS13740 2925389..2925829 + 441 WP_003413460.1 type II toxin-antitoxin system VapC family toxin Toxin
IMW72_RS13745 2925850..2926605 - 756 WP_003413464.1 YebC/PmpR family DNA-binding transcriptional regulator -
IMW72_RS13750 2926738..2927334 - 597 WP_003413465.1 pyridoxal 5'-phosphate synthase glutaminase subunit PdxT -
IMW72_RS13755 2927342..2928187 - 846 WP_003413466.1 acyl-CoA thioesterase II -
IMW72_RS13760 2928216..2929115 - 900 WP_003413468.1 pyridoxal 5'-phosphate synthase lyase subunit PdxS -
IMW72_RS13765 2929243..2929917 + 675 WP_003413471.1 pyridoxamine 5'-phosphate oxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain toxin

(1-146)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 147 a.a.        Molecular weight: 16026.38 Da        Isoelectric Point: 6.8599

>T189870 WP_003413460.1 NZ_CP069078:2925389-2925829 [Mycobacterium tuberculosis]
MLLCDTNIWLALALSGHVHHRASRAWLDTINAPGVIHFCRATQQSLLRLLTNRTVLGAYGSPPLTNREAWAAYAAFLDDD
RIVLAGAEPDGLEAQWRAFAVRQSPAPKVWMDAYLAAFALTGGFELVTTDTAFTQYGGIELRLLAK

Download         Length: 441 bp

>T189870 NZ_CP091022:2348277-2348564 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10453.98 Da        Isoelectric Point: 7.2797

>AT189870 WP_003413456.1 NZ_CP069078:2925115-2925402 [Mycobacterium tuberculosis]
MKTTLDLPDELMRAIKVRAAQQGRKMKDVVTELLRSGLSQTHSGAPIPTPRRVQLPLVHCGGAATREQEMTPERVAAALL
DQEAQWWSGHDDAAL

Download         Length: 288 bp

>AT189870 NZ_CP091022:2348038-2348277 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQK0

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BWB8

References