Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2864772..2865409 Replicon chromosome
Accession NZ_CP069078
Organism Mycobacterium tuberculosis strain N0157

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag IMW72_RS13475 Protein ID WP_003413180.1
Coordinates 2864772..2865167 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag IMW72_RS13480 Protein ID WP_003413183.1
Coordinates 2865164..2865409 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMW72_RS13435 2860175..2861386 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
IMW72_RS13440 2861513..2862172 + 660 WP_003900846.1 LppA family lipoprotein -
IMW72_RS13445 2862169..2862831 + 663 WP_003903936.1 LppA family lipoprotein -
IMW72_RS13450 2862828..2863106 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
IMW72_RS13455 2863199..2863612 + 414 WP_003413164.1 PIN domain nuclease -
IMW72_RS13460 2863651..2863908 + 258 WP_003413167.1 CopG family transcriptional regulator -
IMW72_RS13465 2863905..2864282 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
IMW72_RS13470 2864298..2864672 - 375 WP_003413177.1 hypothetical protein -
IMW72_RS13475 2864772..2865167 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
IMW72_RS13480 2865164..2865409 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
IMW72_RS13485 2865820..2866239 - 420 WP_003413190.1 A24 family peptidase -
IMW72_RS13490 2866251..2867060 - 810 WP_003413193.1 shikimate dehydrogenase -
IMW72_RS13495 2867057..2868310 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
IMW72_RS13500 2868303..2868815 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T189869 WP_003413180.1 NZ_CP069078:c2865167-2864772 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T189869 NZ_CP091022:2060193-2060296 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT189869 WP_003413183.1 NZ_CP069078:c2865409-2865164 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT189869 NZ_CP091022:c2060334-2060066 [Escherichia coli]
AAAGTCAGCGAAGGGAATGTTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References