Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2863651..2864282 Replicon chromosome
Accession NZ_CP069078
Organism Mycobacterium tuberculosis strain N0157

Toxin (Protein)


Gene name vapC Uniprot ID G0TQ28
Locus tag IMW72_RS13465 Protein ID WP_003413174.1
Coordinates 2863905..2864282 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P95006
Locus tag IMW72_RS13460 Protein ID WP_003413167.1
Coordinates 2863651..2863908 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMW72_RS13430 2859565..2859879 + 315 WP_009937839.1 hypothetical protein -
IMW72_RS13435 2860175..2861386 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
IMW72_RS13440 2861513..2862172 + 660 WP_003900846.1 LppA family lipoprotein -
IMW72_RS13445 2862169..2862831 + 663 WP_003903936.1 LppA family lipoprotein -
IMW72_RS13450 2862828..2863106 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
IMW72_RS13455 2863199..2863612 + 414 WP_003413164.1 PIN domain nuclease -
IMW72_RS13460 2863651..2863908 + 258 WP_003413167.1 CopG family transcriptional regulator Antitoxin
IMW72_RS13465 2863905..2864282 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin Toxin
IMW72_RS13470 2864298..2864672 - 375 WP_003413177.1 hypothetical protein -
IMW72_RS13475 2864772..2865167 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 -
IMW72_RS13480 2865164..2865409 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 -
IMW72_RS13485 2865820..2866239 - 420 WP_003413190.1 A24 family peptidase -
IMW72_RS13490 2866251..2867060 - 810 WP_003413193.1 shikimate dehydrogenase -
IMW72_RS13495 2867057..2868310 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
IMW72_RS13500 2868303..2868815 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13728.72 Da        Isoelectric Point: 4.4687

>T189868 WP_003413174.1 NZ_CP069078:2863905-2864282 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Download         Length: 378 bp

>T189868 NZ_CP091022:c1874269-1873892 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCGC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGTCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9453.63 Da        Isoelectric Point: 10.6555

>AT189868 WP_003413167.1 NZ_CP069078:2863651-2863908 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Download         Length: 258 bp

>AT189868 NZ_CP091022:c1874727-1874359 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQ28

Antitoxin

Source ID Structure
AlphaFold DB A0A829C9W5

References