Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC(toxin)
Location 2863199..2863908 Replicon chromosome
Accession NZ_CP069078
Organism Mycobacterium tuberculosis strain N0157

Toxin (Protein)


Gene name vapC Uniprot ID G0TQ26
Locus tag IMW72_RS13455 Protein ID WP_003413164.1
Coordinates 2863199..2863612 (+) Length 138 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P95006
Locus tag IMW72_RS13460 Protein ID WP_003413167.1
Coordinates 2863651..2863908 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMW72_RS13425 2858252..2859457 - 1206 WP_003413027.1 chorismate synthase -
IMW72_RS13430 2859565..2859879 + 315 WP_009937839.1 hypothetical protein -
IMW72_RS13435 2860175..2861386 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
IMW72_RS13440 2861513..2862172 + 660 WP_003900846.1 LppA family lipoprotein -
IMW72_RS13445 2862169..2862831 + 663 WP_003903936.1 LppA family lipoprotein -
IMW72_RS13450 2862828..2863106 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
IMW72_RS13455 2863199..2863612 + 414 WP_003413164.1 PIN domain nuclease Toxin
IMW72_RS13460 2863651..2863908 + 258 WP_003413167.1 CopG family transcriptional regulator Antitoxin
IMW72_RS13465 2863905..2864282 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
IMW72_RS13470 2864298..2864672 - 375 WP_003413177.1 hypothetical protein -
IMW72_RS13475 2864772..2865167 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 -
IMW72_RS13480 2865164..2865409 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 -
IMW72_RS13485 2865820..2866239 - 420 WP_003413190.1 A24 family peptidase -
IMW72_RS13490 2866251..2867060 - 810 WP_003413193.1 shikimate dehydrogenase -
IMW72_RS13495 2867057..2868310 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
IMW72_RS13500 2868303..2868815 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-122)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 138 a.a.        Molecular weight: 15045.00 Da        Isoelectric Point: 5.4673

>T189867 WP_003413164.1 NZ_CP069078:2863199-2863612 [Mycobacterium tuberculosis]
MVFCVDTSAWHHAARPEVARRWLAALSADQIGICDHVRLEILYSANSATDYDALADELDGLARIPVGAETFTRACQVQRE
LAHVAGLHHRSVKIADLVIAAAAELSGTIVWHYDENYDRVAAITGQPTEWIVPRGTL

Download         Length: 414 bp

>T189867 NZ_CP091022:1861208-1861462 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9453.63 Da        Isoelectric Point: 10.6555

>AT189867 WP_003413167.1 NZ_CP069078:2863651-2863908 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Download         Length: 258 bp

>AT189867 NZ_CP091022:1860960-1861211 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQ26

Antitoxin

Source ID Structure
AlphaFold DB A0A829C9W5

References