Detailed information of TA system TA189866

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2849362..2850002
• Replicon: chromosome
• Accession: NZ_CP069078
• Organism: Mycobacterium tuberculosis strain N0157

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: IMW72_RS13370
• Protein ID: WP_003412970.1
• Coordinates: 2849362..2849781 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: IMW72_RS13375
• Protein ID: WP_003412975.1
• Coordinates: 2849778..2850002 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMW72_RS13340	2844947..2845669	-	723	WP_003412957.1	DUF1906 domain-containing protein	-
IMW72_RS13345	2846186..2846413	+	228	WP_003412960.1	type II toxin-antitoxin system VapB family antitoxin	-
IMW72_RS13350	2846410..2846811	+	402	WP_003412963.1	PIN domain-containing protein	-
IMW72_RS13355	2846846..2847766	-	921	WP_003412965.1	restriction endonuclease	-
IMW72_RS13360	2848107..2848313	-	207	WP_229298035.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
IMW72_RS13365	2848411..2849361	+	951	WP_003911916.1	ERCC4 domain-containing protein	-
IMW72_RS13370	2849362..2849781	-	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
IMW72_RS13375	2849778..2850002	-	225	WP_003412975.1	antitoxin VapB39	Antitoxin
IMW72_RS13380	2850033..2852826	-	2794	Protein_2642	amino acid decarboxylase	-
IMW72_RS13385	2852898..2853299	-	402	WP_003412981.1	hypothetical protein	-
IMW72_RS13390	2853299..2853769	-	471	WP_003412985.1	transcription antitermination factor NusB	-
IMW72_RS13395	2853772..2854335	-	564	WP_003412989.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T189866 WP_003412970.1 NZ_CP069078:c2849781-2849362 [Mycobacterium tuberculosis]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T189866 NZ_CP091022:1860960-1861211 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT189866 WP_003412975.1 NZ_CP069078:c2850002-2849778 [Mycobacterium tuberculosis]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT189866 NZ_CP091022:1861208-1861462 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09