Detailed information of TA system TA189862

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2329057..2329693
• Replicon: chromosome
• Accession: NZ_CP069078
• Organism: Mycobacterium tuberculosis strain N0157

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: IMW72_RS10905
• Protein ID: WP_003904769.1
• Coordinates: 2329283..2329693 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: P9WJ84
• Locus tag: IMW72_RS10900
• Protein ID: WP_003410651.1
• Coordinates: 2329057..2329290 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMW72_RS10885	2324514..2324906	+	393	WP_229303073.1	metal ABC transporter permease	-
IMW72_RS10890	2324907..2325311	-	405	WP_003410645.1	PPOX class F420-dependent oxidoreductase	-
IMW72_RS10895	2325395..2328979	-	3585	WP_003410648.1	cobaltochelatase subunit CobN	-
IMW72_RS10900	2329057..2329290	+	234	WP_003410651.1	type II toxin-antitoxin system antitoxin MazE7	Antitoxin
IMW72_RS10905	2329283..2329693	+	411	WP_003904769.1	type II toxin-antitoxin system toxin endoribonuclease MazF7	Toxin
IMW72_RS10910	2329677..2330768	+	1092	WP_003900454.1	precorrin-3B synthase	-
IMW72_RS10915	2330778..2331404	+	627	WP_003410658.1	precorrin-8X methylmutase	-
IMW72_RS10920	2331401..2332873	+	1473	WP_003903738.1	precorrin-2 C(20)-methyltransferase	-
IMW72_RS10925	2332819..2334042	-	1224	WP_003901321.1	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14176.35 Da
• Isoelectric Point: 9.4835

>T189862 WP_003904769.1 NZ_CP069078:2329283-2329693 [Mycobacterium tuberculosis]
MAEPRRGDLWLVSLGAARAGEPGKHRPAVVVSVDELLTGIDDELVVVVPVSSSRSRTPLRPPVAPSEGVAADSVAVCRGV
RAVARARLVERLGALKPATMPAIENALTLILGLPTGPERGEAATHSPVRWTGGRDP

Nucleotide

• Length: 411 bp

>T189862 NZ_CP091022:759149-759445 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCACTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8450.25 Da
• Isoelectric Point: 4.7954

>AT189862 WP_003410651.1 NZ_CP069078:2329057-2329290 [Mycobacterium tuberculosis]
MSTSTTIRVSTQTRDRLAAQARERGISMSALLTELAAQAERQAIFRAEREASHAETTTQAVRDEDREWEGTVGDGLG

Nucleotide

• Length: 234 bp

>AT189862 NZ_CP091022:759447-759842 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A7U4BV50