Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC(toxin)
Location 2227308..2227996 Replicon chromosome
Accession NZ_CP069078
Organism Mycobacterium tuberculosis strain N0157

Toxin (Protein)


Gene name vapC Uniprot ID P0A653
Locus tag IMW72_RS10435 Protein ID WP_003409958.1
Coordinates 2227308..2227727 (-) Length 140 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ28
Locus tag IMW72_RS10440 Protein ID WP_003409968.1
Coordinates 2227736..2227996 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMW72_RS10415 2222803..2223651 + 849 WP_003409942.1 class I SAM-dependent methyltransferase -
IMW72_RS10420 2223614..2225059 - 1446 WP_003409946.1 APC family permease -
IMW72_RS10425 2225238..2225924 - 687 WP_003409954.1 immunoprotective protein Mpt64 -
IMW72_RS10430 2226115..2227083 - 969 WP_003899116.1 class 1b ribonucleoside-diphosphate reductase subunit beta -
IMW72_RS10435 2227308..2227727 - 420 WP_003409958.1 type II toxin-antitoxin system VapC family toxin Toxin
IMW72_RS10440 2227736..2227996 - 261 WP_003409968.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
IMW72_RS10445 2228139..2229788 + 1650 WP_003903703.1 PecA family PE domain-processing aspartic protease -
IMW72_RS10450 2229776..2230429 - 654 WP_003409976.1 cutinase Cfp21 -
IMW72_RS10455 2230544..2230774 + 231 WP_003409980.1 DUF167 domain-containing protein -
IMW72_RS10460 2230859..2231770 - 912 WP_003409986.1 ArgP/LysG family DNA-binding transcriptional regulator -
IMW72_RS10465 2231879..2232478 + 600 WP_003409989.1 L-lysine exporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-125)

Antitoxin

Antitoxin VapB-like

(2-81)


Sequences

Toxin        


Download         Length: 140 a.a.        Molecular weight: 14724.89 Da        Isoelectric Point: 7.9535

>T189857 WP_003409958.1 NZ_CP069078:c2227727-2227308 [Mycobacterium tuberculosis]
MIVDTSAVVALVQGERPHATLVAAALAGAHSPVMSAPTVAECLIVLTARHGPVARTIFERLRSEIGLSVSSFTAEHAAAT
QRAFLRYGKGRHRAALNFGDCMTYATAQLGHQPLLAVGNDFPQTDLEFRGVVGYWPGVA

Download         Length: 420 bp

>T189857 NZ_CP091020:c4711776-4711603 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9528.88 Da        Isoelectric Point: 5.1007

>AT189857 WP_003409968.1 NZ_CP069078:c2227996-2227736 [Mycobacterium tuberculosis]
VALNIKDPEVDRLAAELADRLHTSKTAAIRHALSAQLAFLESRAGDREAQLLDILRTEIWPLLADRSPITKLEREQILGY
DPATGV

Download         Length: 261 bp

>AT189857 NZ_CP091020:c4712100-4711804 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTAATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829C4H9

Antitoxin

Source ID Structure
AlphaFold DB A0A829C483

References