Detailed information of TA system TA189854

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2201949..2202652
• Replicon: chromosome
• Accession: NZ_CP069078
• Organism: Mycobacterium tuberculosis strain N0157

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: IMW72_RS10250
• Protein ID: WP_003409778.1
• Coordinates: 2201949..2202278 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: IMW72_RS10255
• Protein ID: WP_003409780.1
• Coordinates: 2202275..2202652 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMW72_RS10230	2198332..2199402	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
IMW72_RS10235	2199399..2199914	+	516	WP_003409718.1	flavin reductase family protein	-
IMW72_RS10240	2199911..2200972	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
IMW72_RS10245	2200969..2201739	+	771	WP_003409775.1	SDR family oxidoreductase	-
IMW72_RS10250	2201949..2202278	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IMW72_RS10255	2202275..2202652	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
IMW72_RS10260	2202649..2203239	-	591	WP_003409784.1	SEC-C metal-binding domain-containing protein	-
IMW72_RS10265	2203294..2204658	+	1365	WP_003903691.1	HNH endonuclease signature motif containing protein	-
IMW72_RS10270	2204813..2205265	-	453	WP_003899095.1	lipoprotein	-
IMW72_RS10275	2205329..2205730	+	402	WP_003409869.1	hypothetical protein	-
IMW72_RS10280	2205723..2205905	-	183	WP_003409870.1	hypothetical protein	-
IMW72_RS10285	2206019..2206369	-	351	WP_003409871.1	hypothetical protein	-
IMW72_RS10290	2206380..2207282	-	903	WP_003409874.1	hypothetical protein	-
IMW72_RS10295	2207303..2207494	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T189854 WP_003409778.1 NZ_CP069078:c2202278-2201949 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T189854 NZ_CP091020:3885031-3885249 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT189854 WP_003409780.1 NZ_CP069078:c2202652-2202275 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT189854 NZ_CP091020:3884631-3885005 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8