Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 1946579..1947192 Replicon chromosome
Accession NZ_CP069078
Organism Mycobacterium tuberculosis strain N0157

Toxin (Protein)


Gene name vapC Uniprot ID P9WFA2
Locus tag IMW72_RS09105 Protein ID WP_003408465.1
Coordinates 1946579..1946968 (-) Length 130 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ52
Locus tag IMW72_RS09110 Protein ID WP_003408469.1
Coordinates 1946965..1947192 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMW72_RS09075 1941771..1942601 + 831 WP_003907750.1 cyclase family protein -
IMW72_RS09080 1942601..1942951 + 351 WP_003898986.1 cupin domain-containing protein -
IMW72_RS09085 1943004..1943818 + 815 Protein_1792 3-keto-5-aminohexanoate cleavage protein -
IMW72_RS09090 1943832..1944611 + 780 WP_003408460.1 IclR family transcriptional regulator -
IMW72_RS09105 1946579..1946968 - 390 WP_003408465.1 type II toxin-antitoxin system VapC family toxin Toxin
IMW72_RS09110 1946965..1947192 - 228 WP_003408469.1 antitoxin Antitoxin
IMW72_RS09115 1947410..1948894 + 1485 WP_003408473.1 biotin carboxylase -
IMW72_RS09120 1948891..1950138 + 1248 WP_003408476.1 serine hydrolase -
IMW72_RS09125 1950181..1950600 - 420 WP_003408483.1 hypothetical protein -
IMW72_RS09130 1950590..1951300 - 711 WP_003408486.1 winged helix-turn-helix transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-116)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 130 a.a.        Molecular weight: 13817.98 Da        Isoelectric Point: 7.0612

>T189852 WP_003408465.1 NZ_CP069078:c1946968-1946579 [Mycobacterium tuberculosis]
VIVLDASAAVELMLTTPAGAAVARRLRGETVHAPAHFDVEVIGAIRQAVVRQLISDHEGLVVVVNFLSLPVRRWPLKPFT
QRAYQLRSTHTVADGAYVALAEGLGVPLITCDGRLAQSHGHNAEIELVA

Download         Length: 390 bp

>T189852 NZ_CP091020:3003858-3003965 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8226.39 Da        Isoelectric Point: 4.3313

>AT189852 WP_003408469.1 NZ_CP069078:c1947192-1946965 [Mycobacterium tuberculosis]
MSAMVQIRNVPDELLHELKARAAAQRMSLSDFLLARLAEIAEEPALDDVLDRLAALPRRDLGASAAELVDEARSE

Download         Length: 228 bp

>AT189852 NZ_CP091020:c3003811-3003745 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BUI9

Antitoxin

Source ID Structure
PDB 7E4J
AlphaFold DB A0A7U4FAN9

References