Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1683059..1683675 Replicon chromosome
Accession NZ_CP069078
Organism Mycobacterium tuberculosis strain N0157

Toxin (Protein)


Gene name mazF Uniprot ID G0TJ74
Locus tag IMW72_RS07930 Protein ID WP_003407593.1
Coordinates 1683358..1683675 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TJ73
Locus tag IMW72_RS07925 Protein ID WP_003900349.1
Coordinates 1683059..1683361 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMW72_RS07915 1678945..1680792 + 1848 WP_003407585.1 methylmalonyl-CoA mutase small subunit -
IMW72_RS07920 1680793..1683045 + 2253 WP_003407587.1 methylmalonyl-CoA mutase -
IMW72_RS07925 1683059..1683361 + 303 WP_003900349.1 type II toxin-antitoxin system antitoxin MazE4 Antitoxin
IMW72_RS07930 1683358..1683675 + 318 WP_003407593.1 type II toxin-antitoxin system toxin endoribonuclease MazF4 Toxin
IMW72_RS07935 1683672..1684676 + 1005 WP_003407596.1 methylmalonyl Co-A mutase-associated GTPase MeaB -
IMW72_RS07940 1684729..1686018 + 1290 WP_003407599.1 serine hydrolase domain-containing protein -
IMW72_RS07945 1686091..1686708 - 618 WP_003407606.1 class I SAM-dependent methyltransferase -
IMW72_RS07950 1686922..1687134 - 213 WP_003898900.1 dodecin family protein -
IMW72_RS07955 1687195..1687593 + 399 WP_003900351.1 hypothetical protein -
IMW72_RS07960 1687638..1688666 + 1029 WP_003903478.1 glycosyltransferase family 2 protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-99)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 11377.97 Da        Isoelectric Point: 7.9950

>T189850 WP_003407593.1 NZ_CP069078:1683358-1683675 [Mycobacterium tuberculosis]
VNAPLRGQVYRCDLGYGAKPWLIVSNNARNRHTADVVAVRLTTTRRTIPTWVAMGPSDPLTGYVNADNIETLGKDELGDY
LGEVTPATMNKINTALATALGLPWP

Download         Length: 318 bp

>T189850 NZ_CP091020:c2204864-2204670 [Escherichia coli]
ATGCGGTATGAAAAAGTCAAACCATGCCCTTTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
CGCGAAGTGTAACGGATGCGAATCCCGAACCGGCTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGCAATAATAATGGAGGTGTTCATGTATAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11173.42 Da        Isoelectric Point: 9.5564

>AT189850 WP_003900349.1 NZ_CP069078:1683059-1683361 [Mycobacterium tuberculosis]
VPFLVALSGIISGVRDHSMTVRLDQQTRQRLQDIVKGGYRSANAAIVDAINKRWEALHDEQLDAAYAAAIHDNPAYPYES
EAERSAARARRNARQQRSAQ

Download         Length: 303 bp

>AT189850 NZ_CP091020:2204493-2204671 [Escherichia coli]
GATGACAGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTCCCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5XE3
PDB 5XE2

Antitoxin

Source ID Structure
PDB 5XE3
AlphaFold DB G0TJ73

References