Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 1570452..1571107 Replicon chromosome
Accession NZ_CP069078
Organism Mycobacterium tuberculosis strain N0157

Toxin (Protein)


Gene name vapC Uniprot ID A0A7U4FAR1
Locus tag IMW72_RS07430 Protein ID WP_003407268.1
Coordinates 1570452..1570853 (-) Length 134 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TIK2
Locus tag IMW72_RS07435 Protein ID WP_003407272.1
Coordinates 1570850..1571107 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMW72_RS07415 1565927..1567312 - 1386 WP_003407260.1 cytochrome P450 -
IMW72_RS07420 1567390..1568424 + 1035 WP_003407264.1 AraC family transcriptional regulator -
IMW72_RS07425 1568470..1570197 - 1728 Protein_1461 PE family protein -
IMW72_RS07430 1570452..1570853 - 402 WP_003407268.1 type II toxin-antitoxin system VapC family toxin Toxin
IMW72_RS07435 1570850..1571107 - 258 WP_003407272.1 CopG family transcriptional regulator Antitoxin
IMW72_RS07440 1571190..1572149 - 960 WP_003908687.1 alpha/beta hydrolase -
IMW72_RS07445 1572174..1573136 - 963 WP_003407279.1 alpha/beta hydrolase -
IMW72_RS07450 1573135..1573872 + 738 WP_031647000.1 lysoplasmalogenase -
IMW72_RS07455 1573953..1575920 + 1968 WP_003907654.1 primosomal protein N' -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-108)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-39)


Sequences

Toxin        


Download         Length: 134 a.a.        Molecular weight: 14894.39 Da        Isoelectric Point: 11.6897

>T189849 WP_003407268.1 NZ_CP069078:c1570853-1570452 [Mycobacterium tuberculosis]
MILVDSDVLIAHLRGVVAARDWLVSARKDGPLAISVVSTAELIGGMRTAERREVWRLLASFRVQPATEVIARRAGDMMRR
YRRSHNRIGLGDYLIAATADVQGLQLATLNVWHFPMFEQLKPPFAVPGHRPRA

Download         Length: 402 bp

>T189849 NZ_CP091020:2202824-2202927 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTTTTTTT

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9443.58 Da        Isoelectric Point: 6.4631

>AT189849 WP_003407272.1 NZ_CP069078:c1571107-1570850 [Mycobacterium tuberculosis]
MKRTNIYLDEEQTASLDKLAAQEGVSRAELIRLLLNRALTTAGDDLASDLQAINDSFGTLRHLDPPVRRSGGREQHLAQV
WRATS

Download         Length: 258 bp

>AT189849 NZ_CP091020:c2202928-2202694 [Escherichia coli]
TAAAAAAAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCA
TTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAAT
AAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCGTTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FAR1

Antitoxin

Source ID Structure
AlphaFold DB G0TIK2

References