Detailed information of TA system TA189847

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-Phd
• Location: 1381924..1382483
• Replicon: chromosome
• Accession: NZ_CP069078
• Organism: Mycobacterium tuberculosis strain N0157

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G0THS1
• Locus tag: IMW72_RS06610
• Protein ID: WP_003898789.1
• Coordinates: 1381924..1382217 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G0THS2
• Locus tag: IMW72_RS06615
• Protein ID: WP_003406322.1
• Coordinates: 1382214..1382483 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMW72_RS06580	1377122..1378111	+	990	WP_003406301.1	malate dehydrogenase	-
IMW72_RS06585	1378187..1378447	+	261	WP_003406302.1	type II toxin-antitoxin system antitoxin VapB33	-
IMW72_RS06590	1378444..1378875	+	432	WP_003406304.1	type II toxin-antitoxin system VapC family toxin	-
IMW72_RS06595	1378973..1379916	-	944	Protein_1302	PE family protein	-
IMW72_RS06600	1380096..1380956	+	861	WP_003903377.1	glycine betaine ABC transporter substrate-binding protein	-
IMW72_RS06605	1381037..1381867	-	831	WP_003898788.1	SDR family NAD(P)-dependent oxidoreductase	-
IMW72_RS06610	1381924..1382217	-	294	WP_003898789.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
IMW72_RS06615	1382214..1382483	-	270	WP_003406322.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
IMW72_RS06620	1382652..1386347	-	3696	WP_015631286.1	multifunctional oxoglutarate decarboxylase/oxoglutarate dehydrogenase thiamine pyrophosphate-binding subunit/dihydrolipoyllysine-residue succinyltransferase subunit	-
IMW72_RS06625	1386489..1387277	-	789	WP_003903379.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (9-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11031.57 Da
• Isoelectric Point: 9.6275

>T189847 WP_003898789.1 NZ_CP069078:c1382217-1381924 [Mycobacterium tuberculosis]
VSDDHPYHVAITATAARDLQRLPEKIAAACVEFVFGPLLNNPHRLGKPLRNDLEGLHSARRGDYRVVYAIDDGHHRVEII
HIARRSASYRMNPCRPR

Nucleotide

• Length: 294 bp

>T189847 NZ_CP091020:2042516-2042767 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATATAACTCCCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9766.97 Da
• Isoelectric Point: 4.8615

>AT189847 WP_003406322.1 NZ_CP069078:c1382483-1382214 [Mycobacterium tuberculosis]
MAVVPLGEVRNRLSEYVAEVELTHERITITRHGHPAAVLISADDLASIEETLEVLRTPGASEAIREGLADVAAGRFVSND
EIRNRYTAR

Nucleotide

• Length: 270 bp

>AT189847 NZ_CP091020:2042764-2043018 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: G0THS1

Antitoxin

• AlphaFold DB: G0THS2