Detailed information of TA system TA189846

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1378187..1378875
• Replicon: chromosome
• Accession: NZ_CP069078
• Organism: Mycobacterium tuberculosis strain N0157

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0THR7
• Locus tag: IMW72_RS06590
• Protein ID: WP_003406304.1
• Coordinates: 1378444..1378875 (+)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0THR6
• Locus tag: IMW72_RS06585
• Protein ID: WP_003406302.1
• Coordinates: 1378187..1378447 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMW72_RS06565	1373764..1374588	+	825	WP_003900298.1	trehalose ABC transporter permease SugB	-
IMW72_RS06570	1374593..1375774	+	1182	WP_003406299.1	trehalose ABC transporter ATP-binding protein SugC	-
IMW72_RS06575	1375851..1376951	-	1101	WP_003406300.1	magnesium/cobalt transporter CorA	-
IMW72_RS06580	1377122..1378111	+	990	WP_003406301.1	malate dehydrogenase	-
IMW72_RS06585	1378187..1378447	+	261	WP_003406302.1	type II toxin-antitoxin system antitoxin VapB33	Antitoxin
IMW72_RS06590	1378444..1378875	+	432	WP_003406304.1	type II toxin-antitoxin system VapC family toxin	Toxin
IMW72_RS06595	1378973..1379916	-	944	Protein_1302	PE family protein	-
IMW72_RS06600	1380096..1380956	+	861	WP_003903377.1	glycine betaine ABC transporter substrate-binding protein	-
IMW72_RS06605	1381037..1381867	-	831	WP_003898788.1	SDR family NAD(P)-dependent oxidoreductase	-
IMW72_RS06610	1381924..1382217	-	294	WP_003898789.1	type II toxin-antitoxin system mRNA interferase RelE	-
IMW72_RS06615	1382214..1382483	-	270	WP_003406322.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (2-142)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 15873.08 Da
• Isoelectric Point: 5.8838

>T189846 WP_003406304.1 NZ_CP069078:1378444-1378875 [Mycobacterium tuberculosis]
VIIPDINLLLYAVITGFPQHRRAHAWWQDTVNGHTRIGLTYPALFGFLRIATSARVLAAPLPTADAIAYVREWLSQPNVD
LLTAGPRHLDIALGLLDKLGTASHLTTDVQLAAYGIEYDAEIHSSDTDFARFADLKWTDPLRE

Nucleotide

• Length: 432 bp

>T189846 NZ_CP091020:1481430-1481828 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9918.25 Da
• Isoelectric Point: 6.4755

>AT189846 WP_003406302.1 NZ_CP069078:1378187-1378447 [Mycobacterium tuberculosis]
MRTTLTLDDDVVRLVEDAVHRERRPMKQVINDALRRALAPPVKRQEQYRLEPHESAVRSGLDLAGFNKLADELEDEALLD
ATRRAR

Nucleotide

• Length: 261 bp

>AT189846 NZ_CP091020:1481203-1481430 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Toxin

• AlphaFold DB: G0THR7

Antitoxin

• AlphaFold DB: G0THR6