Detailed information of TA system TA189845

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1233319..1233887
• Replicon: chromosome
• Accession: NZ_CP069078
• Organism: Mycobacterium tuberculosis strain N0157

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: IMW72_RS05920
• Protein ID: WP_003405865.1
• Coordinates: 1233513..1233887 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: IMW72_RS05915
• Protein ID: WP_003405863.1
• Coordinates: 1233319..1233516 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMW72_RS05895	1229360..1229998	-	639	WP_003898730.1	lipid droplet-associated protein	-
IMW72_RS05900	1230088..1231095	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
IMW72_RS05905	1231112..1232095	-	984	WP_003903326.1	hypothetical protein	-
IMW72_RS05910	1232158..1233231	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
IMW72_RS05915	1233319..1233516	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
IMW72_RS05920	1233513..1233887	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
IMW72_RS05925	1234090..1234788	+	699	WP_003898733.1	hypothetical protein	-
IMW72_RS05930	1234906..1235091	+	186	WP_003901093.1	hypothetical protein	-
IMW72_RS05935	1235018..1235293	-	276	WP_003405867.1	hypothetical protein	-
IMW72_RS05940	1235536..1235859	+	324	WP_003405871.1	putative quinol monooxygenase	-
IMW72_RS05945	1235874..1236572	-	699	WP_031646953.1	hypothetical protein	-
IMW72_RS05950	1236606..1236815	+	210	WP_003911400.1	hypothetical protein	-
IMW72_RS05955	1236767..1237537	-	771	Protein_1173	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T189845 WP_003405865.1 NZ_CP069078:1233513-1233887 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T189845 NZ_CP091020:1207811-1208122 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT189845 WP_003405863.1 NZ_CP069078:1233319-1233516 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT189845 NZ_CP091020:1208119-1208538 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07