Detailed information of TA system TA189840

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 835608..836317
• Replicon: chromosome
• Accession: NZ_CP069078
• Organism: Mycobacterium tuberculosis strain N0157

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A7U4F979
• Locus tag: IMW72_RS03940
• Protein ID: WP_003903160.1
• Coordinates: 835889..836317 (+)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: IMW72_RS03935
• Protein ID: WP_003903159.1
• Coordinates: 835608..835865 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMW72_RS03930	832732..835517	+	2786	Protein_778	PE family protein	-
IMW72_RS03935	835608..835865	+	258	WP_003903159.1	ribbon-helix-helix protein, CopG family	Antitoxin
IMW72_RS03940	835889..836317	+	429	WP_003903160.1	type II toxin-antitoxin system VapC family toxin	Toxin
IMW72_RS03945	836398..836535	-	138	WP_003403839.1	hypothetical protein	-
IMW72_RS03955	836694..836939	+	246	WP_003403841.1	hypothetical protein	-
IMW72_RS03960	837008..837892	-	885	WP_003403844.1	3-hydroxyisobutyrate dehydrogenase	-
IMW72_RS03965	837903..839075	-	1173	WP_003403845.1	acyl-CoA dehydrogenase family protein	-
IMW72_RS03970	839082..840614	-	1533	WP_003403847.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-133)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-36)

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15799.21 Da
• Isoelectric Point: 7.5536

>T189840 WP_003903160.1 NZ_CP069078:835889-836317 [Mycobacterium tuberculosis]
MFLLDANVLLAAHRGDHPNHRTVRPWFDRLLAADDPFTVPNLVWASFLRLATNRRIFEIPSPRAEAFAFVEAVTAQPHHL
PTNPGPRHLVLLRKLCDEADASGDLIPDAVLAAIAVGHHCAVVSLDRDFARFASVRHIRPPL

Nucleotide

• Length: 429 bp

>T189840 NZ_CP091020:c610642-610178 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9142.33 Da
• Isoelectric Point: 10.6530

>AT189840 WP_003903159.1 NZ_CP069078:835608-835865 [Mycobacterium tuberculosis]
MRTTVSISDEILAAAKRRARERGQSLGAVIEDALRREFAAAHVGGARPTVPVFDGGTGPRRGIDLTSNRVLSEVLDEGLE
LNSRK

Nucleotide

• Length: 258 bp

>AT189840 NZ_CP091020:c610977-610642 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A7U4F979