Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapC-mazE/PIN(toxin)
Location 749264..750056 Replicon chromosome
Accession NZ_CP069078
Organism Mycobacterium tuberculosis strain N0157

Toxin (Protein)


Gene name vapC Uniprot ID P9WFB2
Locus tag IMW72_RS03460 Protein ID WP_003403386.1
Coordinates 749619..750056 (-) Length 146 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TQE0
Locus tag IMW72_RS03455 Protein ID WP_003403381.1
Coordinates 749264..749509 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMW72_RS03425 744284..745789 + 1506 WP_003403360.1 carotenoid cleavage oxygenase -
IMW72_RS03430 745870..746880 + 1011 WP_031653722.1 ABC transporter ATP-binding protein -
IMW72_RS03435 747268..747651 - 384 WP_003403365.1 ribonuclease VapC6 -
IMW72_RS03440 747746..747901 - 156 WP_003403368.1 type II toxin-antitoxin system VapB family antitoxin -
IMW72_RS03445 747977..748693 - 717 WP_003903122.1 CPBP family intramembrane metalloprotease -
IMW72_RS03450 748969..749277 - 309 WP_003403376.1 type II toxin-antitoxin system PemK/MazF family toxin -
IMW72_RS03455 749264..749509 - 246 WP_003403381.1 ribbon-helix-helix protein, CopG family Antitoxin
IMW72_RS03460 749619..750056 - 438 WP_003403386.1 type II toxin-antitoxin system VapC family toxin Toxin
IMW72_RS03465 750053..750307 - 255 WP_003911263.1 antitoxin VapB7 -
IMW72_RS03470 750421..752784 + 2364 WP_003903123.1 arylsulfatase AtsD -
IMW72_RS03475 752847..753173 + 327 WP_003403401.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
IMW72_RS03480 753085..753423 + 339 WP_003403405.1 PIN domain-containing protein -
IMW72_RS03485 753420..753593 + 174 WP_003898549.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-126)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 146 a.a.        Molecular weight: 15245.39 Da        Isoelectric Point: 5.1865

>T189838 WP_003403386.1 NZ_CP069078:c750056-749619 [Mycobacterium tuberculosis]
MIVLDTTVLVYAKGAEHPLRDPCRDLVAAIADERIAATTTAEVIQEFVHVRARRRDRSDAAALGRVTMPNCSRRYSPSIE
ATSKRGLTLFETTPGLEACDAVLAAVAASAGATALVSADPAFADLSDVVHVIPDAAGMVSLLGDR

Download         Length: 438 bp

>T189838 NZ_CP091020:272189-272419 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9169.01 Da        Isoelectric Point: 4.2070

>AT189838 WP_003403381.1 NZ_CP069078:c749509-749264 [Mycobacterium tuberculosis]
MLSFRADDHDVDLADAWARRLHIGRSELLRDALRRHLAALAADQDVQAYTERPLTDDENALAEIADWGPAEDWADWADAA
R

Download         Length: 246 bp

>AT189838 NZ_CP091020:272416-272775 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BSW5

Antitoxin

Source ID Structure
AlphaFold DB G0TQE0

References