Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 747268..747901 Replicon chromosome
Accession NZ_CP069078
Organism Mycobacterium tuberculosis strain N0157

Toxin (Protein)


Gene name vapC Uniprot ID G0TQD6
Locus tag IMW72_RS03435 Protein ID WP_003403365.1
Coordinates 747268..747651 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ56
Locus tag IMW72_RS03440 Protein ID WP_003403368.1
Coordinates 747746..747901 (-) Length 52 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMW72_RS03410 742560..743096 + 537 WP_003403341.1 50S ribosomal protein L10 -
IMW72_RS03415 743133..743525 + 393 WP_003403353.1 50S ribosomal protein L7/L12 -
IMW72_RS03420 743518..744213 - 696 WP_003403355.1 TetR/AcrR family transcriptional regulator -
IMW72_RS03425 744284..745789 + 1506 WP_003403360.1 carotenoid cleavage oxygenase -
IMW72_RS03430 745870..746880 + 1011 WP_031653722.1 ABC transporter ATP-binding protein -
IMW72_RS03435 747268..747651 - 384 WP_003403365.1 ribonuclease VapC6 Toxin
IMW72_RS03440 747746..747901 - 156 WP_003403368.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
IMW72_RS03445 747977..748693 - 717 WP_003903122.1 CPBP family intramembrane metalloprotease -
IMW72_RS03450 748969..749277 - 309 WP_003403376.1 type II toxin-antitoxin system PemK/MazF family toxin -
IMW72_RS03455 749264..749509 - 246 WP_003403381.1 ribbon-helix-helix protein, CopG family -
IMW72_RS03460 749619..750056 - 438 WP_003403386.1 type II toxin-antitoxin system VapC family toxin -
IMW72_RS03465 750053..750307 - 255 WP_003911263.1 antitoxin VapB7 -
IMW72_RS03470 750421..752784 + 2364 WP_003903123.1 arylsulfatase AtsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Bacterial antitoxin of type II TA system, VapB

(3-44)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13959.73 Da        Isoelectric Point: 6.0803

>T189836 WP_003403365.1 NZ_CP069078:c747651-747268 [Mycobacterium tuberculosis]
MAAATTTGTHRGLELRAAQRAVGSCEPQRAEFCRSARNADEFDQMSRMFGDVYPDVPVPKSVWRWIDSAQHRLARAGAVG
ALSVVDLLICDTAAARGLVVLHDDADYELAERHLPDIRVRRVVSADD

Download         Length: 384 bp

>T189836 NZ_CP091018:c4711780-4711607 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 52 a.a.        Molecular weight: 5872.72 Da        Isoelectric Point: 5.7734

>AT189836 WP_003403368.1 NZ_CP069078:c747901-747746 [Mycobacterium tuberculosis]
VSVTQIDLDDEALADVMRIAAVHTKKEAVNLAMRDYVERFRRIEALARSRE

Download         Length: 156 bp

>AT189836 NZ_CP091018:c4712104-4711808 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTAATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQD6

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BSF8

References