Detailed information of TA system TA189824

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Rv0298-Rv0299/-
• Location: 366090..366616
• Replicon: chromosome
• Accession: NZ_CP069078
• Organism: Mycobacterium tuberculosis strain N0157

Toxin (Protein)

• Gene name: Rv0299
• Uniprot ID: -
• Locus tag: IMW72_RS01585
• Protein ID: WP_003401560.1
• Coordinates: 366314..366616 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: Rv0298
• Uniprot ID: P9WJ08
• Locus tag: IMW72_RS01580
• Protein ID: WP_003401555.1
• Coordinates: 366090..366317 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMW72_RS01560	361138..361923	+	786	WP_003401538.1	trans-aconitate 2-methyltransferase	-
IMW72_RS01565	361912..362715	-	804	WP_003401540.1	Stf0 family sulphotransferase	-
IMW72_RS01570	362725..364122	-	1398	Protein_311	sulfatase	-
IMW72_RS01575	364301..365947	+	1647	WP_031654185.1	PE family protein	-
IMW72_RS01580	366090..366317	+	228	WP_003401555.1	ribbon-helix-helix protein, CopG family	Antitoxin
IMW72_RS01585	366314..366616	+	303	WP_003401560.1	toxin-antitoxin system toxin	Toxin
IMW72_RS01590	366664..366885	+	222	WP_003401563.1	type II toxin-antitoxin system antitoxin VapB2	-
IMW72_RS01595	366882..367307	+	426	WP_003401566.1	PIN domain nuclease	-
IMW72_RS01600	367443..368075	+	633	WP_003401571.1	TetR/AcrR family transcriptional regulator	-
IMW72_RS01605	368072..368980	+	909	WP_003900117.1	protochlorophyllide reductase	-
IMW72_RS01610	368988..369578	-	591	WP_244750106.1	hypothetical protein	-
IMW72_RS01615	369571..369621	-	51	Protein_320	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Ribbon-helix-helix protein, CopG (6-41)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 10442.16 Da
• Isoelectric Point: 4.9609

>T189824 WP_003401560.1 NZ_CP069078:366314-366616 [Mycobacterium tuberculosis]
MIAPGDIAPRRDSEHELYVAVLSNALHRAADTGRVITCPFIPGRVPEDLLAMVVAVEQPNGTLLPELVQWLHVAALGAPL
GNAGVAALREAASVVTALLC

Nucleotide

• Length: 303 bp

>T189824 NZ_CP091018:1207816-1208127 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8130.21 Da
• Isoelectric Point: 7.0084

>AT189824 WP_003401555.1 NZ_CP069078:366090-366317 [Mycobacterium tuberculosis]
MTKEKISVTVDAAVLAAIDADARAAGLNRSEMIEQALRNEHLRVALRDYTAKTVPALDIDAYAQRVYQANRAAGS

Nucleotide

• Length: 228 bp

>AT189824 NZ_CP091018:1208124-1208543 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A7U4F8T1