Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/Txe-YefM
Location 3779635..3780164 Replicon chromosome
Accession NZ_CP069077
Organism Mycobacterium tuberculosis strain N0072

Toxin (Protein)


Gene name relE Uniprot ID G0TI95
Locus tag JN135_RS17730 Protein ID WP_003417760.1
Coordinates 3779907..3780164 (+) Length 86 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G0TI94
Locus tag JN135_RS17725 Protein ID WP_003417757.1
Coordinates 3779635..3779910 (+) Length 92 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN135_RS17690 3776208..3776897 - 690 WP_003917774.1 BBE domain-containing protein -
JN135_RS17695 3777084..3777455 - 372 WP_003417739.1 FAD-binding protein -
JN135_RS17700 3777353..3777571 - 219 WP_157132559.1 hypothetical protein -
JN135_RS17705 3777598..3777858 - 261 WP_003417741.1 hypothetical protein -
JN135_RS17710 3777973..3778362 + 390 WP_003417745.1 DUF732 domain-containing protein -
JN135_RS17715 3778376..3778669 - 294 WP_003417749.1 DUF3017 domain-containing protein -
JN135_RS17720 3778666..3779511 - 846 WP_003905054.1 bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase -
JN135_RS17725 3779635..3779910 + 276 WP_003417757.1 type II toxin-antitoxin system antitoxin RelJ Antitoxin
JN135_RS17730 3779907..3780164 + 258 WP_003417760.1 Txe/YoeB family addiction module toxin Toxin
JN135_RS17735 3780206..3781396 + 1191 WP_003900033.1 NADH:flavin oxidoreductase -
JN135_RS17740 3781513..3781881 + 369 WP_003417765.1 FHA domain-containing protein -
JN135_RS17745 3781878..3782429 - 552 WP_003417767.1 pentapeptide repeat protein MfpA -
JN135_RS17750 3782436..3783017 - 582 WP_015631560.1 ATP/GTP-binding protein -
JN135_RS17755 3782998..3783366 - 369 WP_003417772.1 DUF742 domain-containing protein -
JN135_RS17760 3783344..3783736 - 393 WP_003417776.1 serine protease inhibitor -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(6-85)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(2-72)


Sequences

Toxin        


Download         Length: 86 a.a.        Molecular weight: 10070.30 Da        Isoelectric Point: 6.4693

>T189820 WP_003417760.1 NZ_CP069077:3779907-3780164 [Mycobacterium tuberculosis]
VRSVNFDPDAWEDFLFWLAADRKTARRITRLIGEIQRDPFSGIGKPEPLQGELSGYWSRRIDDEHRLVYRAGDDEVTMLK
ARYHY

Download         Length: 258 bp

>T189820 NZ_CP091018:740163-740459 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCACTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin


Download         Length: 92 a.a.        Molecular weight: 10194.35 Da        Isoelectric Point: 4.6652

>AT189820 WP_003417757.1 NZ_CP069077:3779635-3779910 [Mycobacterium tuberculosis]
MSISASEARQRLFPLIEQVNTDHQPVRITSRAGDAVLMSADDYDAWQETVYLLRSPENARRLMEAVARDKAGHSAFTKSV
DELREMAGGEE

Download         Length: 276 bp

>AT189820 NZ_CP091018:740461-740856 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3OEI

Antitoxin

Source ID Structure
PDB 3CTO
PDB 3OEI
PDB 3D55
AlphaFold DB G0TI94

References