Detailed information of TA system TA189820

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/Txe-YefM
• Location: 3779635..3780164
• Replicon: chromosome
• Accession: NZ_CP069077
• Organism: Mycobacterium tuberculosis strain N0072

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G0TI95
• Locus tag: JN135_RS17730
• Protein ID: WP_003417760.1
• Coordinates: 3779907..3780164 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G0TI94
• Locus tag: JN135_RS17725
• Protein ID: WP_003417757.1
• Coordinates: 3779635..3779910 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN135_RS17690	3776208..3776897	-	690	WP_003917774.1	BBE domain-containing protein	-
JN135_RS17695	3777084..3777455	-	372	WP_003417739.1	FAD-binding protein	-
JN135_RS17700	3777353..3777571	-	219	WP_157132559.1	hypothetical protein	-
JN135_RS17705	3777598..3777858	-	261	WP_003417741.1	hypothetical protein	-
JN135_RS17710	3777973..3778362	+	390	WP_003417745.1	DUF732 domain-containing protein	-
JN135_RS17715	3778376..3778669	-	294	WP_003417749.1	DUF3017 domain-containing protein	-
JN135_RS17720	3778666..3779511	-	846	WP_003905054.1	bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase	-
JN135_RS17725	3779635..3779910	+	276	WP_003417757.1	type II toxin-antitoxin system antitoxin RelJ	Antitoxin
JN135_RS17730	3779907..3780164	+	258	WP_003417760.1	Txe/YoeB family addiction module toxin	Toxin
JN135_RS17735	3780206..3781396	+	1191	WP_003900033.1	NADH:flavin oxidoreductase	-
JN135_RS17740	3781513..3781881	+	369	WP_003417765.1	FHA domain-containing protein	-
JN135_RS17745	3781878..3782429	-	552	WP_003417767.1	pentapeptide repeat protein MfpA	-
JN135_RS17750	3782436..3783017	-	582	WP_015631560.1	ATP/GTP-binding protein	-
JN135_RS17755	3782998..3783366	-	369	WP_003417772.1	DUF742 domain-containing protein	-
JN135_RS17760	3783344..3783736	-	393	WP_003417776.1	serine protease inhibitor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (6-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-72)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10070.30 Da
• Isoelectric Point: 6.4693

>T189820 WP_003417760.1 NZ_CP069077:3779907-3780164 [Mycobacterium tuberculosis]
VRSVNFDPDAWEDFLFWLAADRKTARRITRLIGEIQRDPFSGIGKPEPLQGELSGYWSRRIDDEHRLVYRAGDDEVTMLK
ARYHY

Nucleotide

• Length: 258 bp

>T189820 NZ_CP091018:740163-740459 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCACTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10194.35 Da
• Isoelectric Point: 4.6652

>AT189820 WP_003417757.1 NZ_CP069077:3779635-3779910 [Mycobacterium tuberculosis]
MSISASEARQRLFPLIEQVNTDHQPVRITSRAGDAVLMSADDYDAWQETVYLLRSPENARRLMEAVARDKAGHSAFTKSV
DELREMAGGEE

Nucleotide

• Length: 276 bp

>AT189820 NZ_CP091018:740461-740856 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• PDB: 3OEI

Antitoxin

• AlphaFold DB: G0TI94