Detailed information of TA system TA189819

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3711197..3711871
• Replicon: chromosome
• Accession: NZ_CP069077
• Organism: Mycobacterium tuberculosis strain N0072

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF52
• Locus tag: JN135_RS17515
• Protein ID: WP_003417282.1
• Coordinates: 3711197..3711625 (-)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TI59
• Locus tag: JN135_RS17520
• Protein ID: WP_003417286.1
• Coordinates: 3711629..3711871 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN135_RS17490	3707019..3707420	-	402	WP_003417264.1	cytidine deaminase	-
JN135_RS17495	3707657..3707995	+	339	WP_003904248.1	succinate dehydrogenase, cytochrome b556 subunit	-
JN135_RS17500	3707992..3708426	+	435	WP_003417269.1	succinate dehydrogenase hydrophobic membrane anchor subunit	-
JN135_RS17505	3708555..3710327	+	1773	WP_003417273.1	succinate dehydrogenase flavoprotein subunit	-
JN135_RS17510	3710327..3711118	+	792	WP_003417279.1	succinate dehydrogenase iron-sulfur subunit	-
JN135_RS17515	3711197..3711625	-	429	WP_003417282.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN135_RS17520	3711629..3711871	-	243	WP_003417286.1	CopG family transcriptional regulator	Antitoxin
JN135_RS17525	3711993..3712607	-	615	WP_003417288.1	class I SAM-dependent methyltransferase	-
JN135_RS17530	3712604..3713269	-	666	WP_003417290.1	molybdopterin converting factor subunit 1	-
JN135_RS17535	3713270..3713803	-	534	WP_003417293.1	cyclic pyranopterin monophosphate synthase MoaC	-
JN135_RS17540	3713800..3714174	-	375	WP_003417295.1	4a-hydroxytetrahydrobiopterin dehydratase	-
JN135_RS17545	3714271..3715275	-	1005	WP_003914475.1	GTP 3',8-cyclase MoaA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-141)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15834.16 Da
• Isoelectric Point: 8.5471

>T189819 WP_003417282.1 NZ_CP069077:c3711625-3711197 [Mycobacterium tuberculosis]
MRALLDVNVLLALLDRDHVDHERARAWITGQIERGWASCAITQNGFVRVISQPRYPSPISVAHAIDLLARATHTRYHEFW
SCTVSILDSKVIDRSRLHSPKQVTDAYLLALAVAHDGRFVTFDQSIALTAVPGATKQHLATL

Nucleotide

• Length: 429 bp

>T189819 NZ_CP091018:c610642-610178 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8849.86 Da
• Isoelectric Point: 5.1007

>AT189819 WP_003417286.1 NZ_CP069077:c3711871-3711629 [Mycobacterium tuberculosis]
MRTTLSIDDDVLLAVKERARREKRTAGEILSDLARQALTNQNPQPAASQEDAFHGFEPLPHRGGAVSNALIDRLRDEEAV

Nucleotide

• Length: 243 bp

>AT189819 NZ_CP091018:c610977-610642 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A7U4FBL0

Antitoxin

• AlphaFold DB: G0TI59