Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN_Sll0205-like-Phd
Location 3143404..3144008 Replicon chromosome
Accession NZ_CP069077
Organism Mycobacterium tuberculosis strain N0072

Toxin (Protein)


Gene name vapC Uniprot ID P71623
Locus tag JN135_RS14945 Protein ID WP_003414492.1
Coordinates 3143404..3143796 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A829CBY8
Locus tag JN135_RS14950 Protein ID WP_003414495.1
Coordinates 3143793..3144008 (-) Length 72 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN135_RS14915 3138554..3139342 - 789 WP_003917092.1 CRISPR-associated endoribonuclease Cas6 -
JN135_RS14920 3139676..3140221 - 546 WP_003904931.1 DUF1802 family protein -
JN135_RS14925 3140493..3141377 - 885 WP_003414409.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
JN135_RS14930 3141380..3142267 - 888 WP_003414414.1 type IV toxin-antitoxin system AbiEi family antitoxin -
JN135_RS14935 3142572..3143117 - 546 WP_003904931.1 DUF1802 family protein -
JN135_RS14940 3143114..3143383 - 270 WP_003414489.1 DUF2277 family protein -
JN135_RS14945 3143404..3143796 - 393 WP_003414492.1 type II toxin-antitoxin system VapC family toxin Toxin
JN135_RS14950 3143793..3144008 - 216 WP_003414495.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
JN135_RS14955 3144055..3144804 + 750 WP_003414497.1 enoyl-CoA hydratase -
JN135_RS14960 3144883..3145965 - 1083 WP_003414499.1 sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC -
JN135_RS14965 3145958..3147268 - 1311 WP_003414501.1 ABC transporter substrate-binding protein -
JN135_RS14970 3147271..3148098 - 828 WP_003414504.1 carbohydrate ABC transporter permease -
JN135_RS14975 3148095..3149006 - 912 WP_003414505.1 sugar ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

VapC-like Sll0205 protein, PIN domain

(5-130)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(2-46)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14610.74 Da        Isoelectric Point: 6.7594

>T189815 WP_003414492.1 NZ_CP069077:c3143796-3143404 [Mycobacterium tuberculosis]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Download         Length: 393 bp

>T189815 NZ_CP091016:c4711746-4711573 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 72 a.a.        Molecular weight: 7511.55 Da        Isoelectric Point: 4.9732

>AT189815 WP_003414495.1 NZ_CP069077:c3144008-3143793 [Mycobacterium tuberculosis]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Download         Length: 216 bp

>AT189815 NZ_CP091016:c4712070-4711774 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTAATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BWM8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CBY8

References