Detailed information of TA system TA189812

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3076298..3076985
• Replicon: chromosome
• Accession: NZ_CP069077
• Organism: Mycobacterium tuberculosis strain N0072

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TF65
• Locus tag: JN135_RS14580
• Protein ID: WP_003414064.1
• Coordinates: 3076590..3076985 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: JN135_RS14575
• Protein ID: WP_003414061.1
• Coordinates: 3076298..3076564 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN135_RS14545	3071937..3072839	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
JN135_RS14550	3072908..3073660	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
JN135_RS14560	3073904..3074179	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
JN135_RS14565	3074176..3075798	-	1623	WP_003414057.1	class I SAM-dependent DNA methyltransferase	-
JN135_RS14570	3075885..3076301	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	-
JN135_RS14575	3076298..3076564	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JN135_RS14580	3076590..3076985	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN135_RS14585	3076982..3077251	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
JN135_RS14590	3077261..3078355	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
JN135_RS14595	3078352..3078771	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
JN135_RS14600	3078770..3078844	+	75	Protein_2881	hypothetical protein	-
JN135_RS14605	3078845..3079324	-	480	WP_003414073.1	dihydrofolate reductase	-
JN135_RS14610	3079395..3080195	-	801	WP_031680801.1	thymidylate synthase	-
JN135_RS14615	3080351..3081088	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-125)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14340.04 Da
• Isoelectric Point: 4.7145

>T189812 WP_003414064.1 NZ_CP069077:c3076985-3076590 [Mycobacterium tuberculosis]
VIVDTSAIVAIVSGESGAQVLKEALERSPNSRMSAPNYVELCAIMQRRDRPEISRLVDRLLDDYGIQVEAVDADQARVAA
QAYRDYGRGSGHPARLNLGDTYSYALAQVTGEPLLFRGDDFTHTDIRPACT

Nucleotide

• Length: 396 bp

>T189812 NZ_CP091016:3885025-3885243 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT189812 WP_003414061.1 NZ_CP069077:c3076564-3076298 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT189812 NZ_CP091016:3884625-3884999 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: G0TF65

Antitoxin

• AlphaFold DB: G0TF64