Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 3075885..3076564 Replicon chromosome
Accession NZ_CP069077
Organism Mycobacterium tuberculosis strain N0072

Toxin (Protein)


Gene name vapC Uniprot ID P9WF90
Locus tag JN135_RS14570 Protein ID WP_003414059.1
Coordinates 3075885..3076301 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TF64
Locus tag JN135_RS14575 Protein ID WP_003414061.1
Coordinates 3076298..3076564 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN135_RS14545 3071937..3072839 - 903 WP_003900564.1 4-hydroxy-tetrahydrodipicolinate synthase -
JN135_RS14550 3072908..3073660 - 753 WP_003899465.1 FAD-dependent thymidylate synthase -
JN135_RS14560 3073904..3074179 - 276 WP_003414055.1 type I restriction endonuclease subunit S -
JN135_RS14565 3074176..3075798 - 1623 WP_003414057.1 class I SAM-dependent DNA methyltransferase -
JN135_RS14570 3075885..3076301 - 417 WP_003414059.1 type II toxin-antitoxin system toxin ribonuclease C21 Toxin
JN135_RS14575 3076298..3076564 - 267 WP_003414061.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
JN135_RS14580 3076590..3076985 - 396 WP_003414064.1 type II toxin-antitoxin system VapC family toxin -
JN135_RS14585 3076982..3077251 - 270 WP_003414066.1 type II toxin-antitoxin system VapB family antitoxin -
JN135_RS14590 3077261..3078355 - 1095 WP_003414068.1 restriction endonuclease subunit S -
JN135_RS14595 3078352..3078771 - 420 WP_003414070.1 winged helix-turn-helix domain-containing protein -
JN135_RS14600 3078770..3078844 + 75 Protein_2881 hypothetical protein -
JN135_RS14605 3078845..3079324 - 480 WP_003414073.1 dihydrofolate reductase -
JN135_RS14610 3079395..3080195 - 801 WP_031680801.1 thymidylate synthase -
JN135_RS14615 3080351..3081088 + 738 WP_003414079.1 dienelactone hydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-123)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(16-56)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15773.00 Da        Isoelectric Point: 7.1294

>T189811 WP_003414059.1 NZ_CP069077:c3076301-3075885 [Mycobacterium tuberculosis]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Download         Length: 417 bp

>T189811 NZ_CP091016:3004388-3004495 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9493.77 Da        Isoelectric Point: 7.2783

>AT189811 WP_003414061.1 NZ_CP069077:c3076564-3076298 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Download         Length: 267 bp

>AT189811 NZ_CP091016:c3004335-3004274 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5SV2

Antitoxin

Source ID Structure
AlphaFold DB G0TF64

References