Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2946759..2947473 Replicon chromosome
Accession NZ_CP069077
Organism Mycobacterium tuberculosis strain N0072

Toxin (Protein)


Gene name vapC Uniprot ID G0TQK0
Locus tag JN135_RS13815 Protein ID WP_003413460.1
Coordinates 2947033..2947473 (+) Length 147 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ20
Locus tag JN135_RS13810 Protein ID WP_003413456.1
Coordinates 2946759..2947046 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN135_RS13775 2942181..2942426 + 246 WP_003413429.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
JN135_RS13780 2942423..2942827 + 405 WP_003413432.1 type II toxin-antitoxin system VapC family toxin -
JN135_RS13785 2943044..2943664 + 621 WP_003413441.1 DUF4178 domain-containing protein -
JN135_RS13790 2943675..2944169 + 495 WP_003413444.1 DUF2617 family protein -
JN135_RS13795 2944166..2944597 + 432 WP_003413445.1 DUF4247 domain-containing protein -
JN135_RS13800 2944622..2945080 + 459 WP_003413451.1 DUF350 domain-containing protein -
JN135_RS13805 2945077..2946648 + 1572 WP_003899392.1 polyamine aminopropyltransferase -
JN135_RS13810 2946759..2947046 + 288 WP_003413456.1 antitoxin VapB41 Antitoxin
JN135_RS13815 2947033..2947473 + 441 WP_003413460.1 type II toxin-antitoxin system VapC family toxin Toxin
JN135_RS13820 2947494..2948249 - 756 WP_003413464.1 YebC/PmpR family DNA-binding transcriptional regulator -
JN135_RS13825 2948382..2948978 - 597 WP_003413465.1 pyridoxal 5'-phosphate synthase glutaminase subunit PdxT -
JN135_RS13830 2948986..2949831 - 846 WP_003413466.1 acyl-CoA thioesterase II -
JN135_RS13835 2949860..2950759 - 900 WP_003413468.1 pyridoxal 5'-phosphate synthase lyase subunit PdxS -
JN135_RS13840 2950887..2951561 + 675 WP_003413471.1 pyridoxamine 5'-phosphate oxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain toxin

(1-146)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 147 a.a.        Molecular weight: 16026.38 Da        Isoelectric Point: 6.8599

>T189810 WP_003413460.1 NZ_CP069077:2947033-2947473 [Mycobacterium tuberculosis]
MLLCDTNIWLALALSGHVHHRASRAWLDTINAPGVIHFCRATQQSLLRLLTNRTVLGAYGSPPLTNREAWAAYAAFLDDD
RIVLAGAEPDGLEAQWRAFAVRQSPAPKVWMDAYLAAFALTGGFELVTTDTAFTQYGGIELRLLAK

Download         Length: 441 bp

>T189810 NZ_CP091016:3003852-3003959 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10453.98 Da        Isoelectric Point: 7.2797

>AT189810 WP_003413456.1 NZ_CP069077:2946759-2947046 [Mycobacterium tuberculosis]
MKTTLDLPDELMRAIKVRAAQQGRKMKDVVTELLRSGLSQTHSGAPIPTPRRVQLPLVHCGGAATREQEMTPERVAAALL
DQEAQWWSGHDDAAL

Download         Length: 288 bp

>AT189810 NZ_CP091016:c3003805-3003739 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQK0

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BWB8

References