Detailed information of TA system TA189806

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2870949..2871589
• Replicon: chromosome
• Accession: NZ_CP069077
• Organism: Mycobacterium tuberculosis strain N0072

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: JN135_RS13445
• Protein ID: WP_003412970.1
• Coordinates: 2870949..2871368 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: JN135_RS13450
• Protein ID: WP_003412975.1
• Coordinates: 2871365..2871589 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN135_RS13415	2866534..2867256	-	723	WP_003412957.1	DUF1906 domain-containing protein	-
JN135_RS13420	2867773..2868000	+	228	WP_003412960.1	type II toxin-antitoxin system VapB family antitoxin	-
JN135_RS13425	2867997..2868398	+	402	WP_003412963.1	PIN domain-containing protein	-
JN135_RS13430	2868433..2869353	-	921	WP_003412965.1	restriction endonuclease	-
JN135_RS13435	2869694..2869900	-	207	WP_229298035.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
JN135_RS13440	2869998..2870948	+	951	WP_003911916.1	ERCC4 domain-containing protein	-
JN135_RS13445	2870949..2871368	-	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN135_RS13450	2871365..2871589	-	225	WP_003412975.1	antitoxin VapB39	Antitoxin
JN135_RS13455	2871621..2874464	-	2844	WP_003904863.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
JN135_RS13460	2874536..2874937	-	402	WP_003412981.1	hypothetical protein	-
JN135_RS13465	2874937..2875407	-	471	WP_003412985.1	transcription antitermination factor NusB	-
JN135_RS13470	2875410..2875973	-	564	WP_003412989.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T189806 WP_003412970.1 NZ_CP069077:c2871368-2870949 [Mycobacterium tuberculosis]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T189806 NZ_CP091016:2042758-2043012 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT189806 WP_003412975.1 NZ_CP069077:c2871589-2871365 [Mycobacterium tuberculosis]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT189806 NZ_CP091016:2042510-2042761 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATATAACTCCCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09