Detailed information of TA system TA189802

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2342194..2342830
• Replicon: chromosome
• Accession: NZ_CP069077
• Organism: Mycobacterium tuberculosis strain N0072

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: JN135_RS10960
• Protein ID: WP_003904769.1
• Coordinates: 2342420..2342830 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: P9WJ84
• Locus tag: JN135_RS10955
• Protein ID: WP_003410651.1
• Coordinates: 2342194..2342427 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN135_RS10940	2337651..2338043	+	393	WP_229303073.1	metal ABC transporter permease	-
JN135_RS10945	2338044..2338448	-	405	WP_003410645.1	PPOX class F420-dependent oxidoreductase	-
JN135_RS10950	2338532..2342116	-	3585	WP_003410648.1	cobaltochelatase subunit CobN	-
JN135_RS10955	2342194..2342427	+	234	WP_003410651.1	type II toxin-antitoxin system antitoxin MazE7	Antitoxin
JN135_RS10960	2342420..2342830	+	411	WP_003904769.1	type II toxin-antitoxin system toxin endoribonuclease MazF7	Toxin
JN135_RS10965	2342814..2343905	+	1092	WP_003900454.1	precorrin-3B synthase	-
JN135_RS10970	2343915..2344541	+	627	WP_031680792.1	precorrin-8X methylmutase	-
JN135_RS10975	2344538..2346064	+	1527	WP_003410659.1	precorrin-2 C(20)-methyltransferase	-
JN135_RS10980	2346010..2347233	-	1224	WP_003901321.1	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14176.35 Da
• Isoelectric Point: 9.4835

>T189802 WP_003904769.1 NZ_CP069077:2342420-2342830 [Mycobacterium tuberculosis]
MAEPRRGDLWLVSLGAARAGEPGKHRPAVVVSVDELLTGIDDELVVVVPVSSSRSRTPLRPPVAPSEGVAADSVAVCRGV
RAVARARLVERLGALKPATMPAIENALTLILGLPTGPERGEAATHSPVRWTGGRDP

Nucleotide

• Length: 411 bp

>T189802 NZ_CP091016:1136953-1137288 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8450.25 Da
• Isoelectric Point: 4.7954

>AT189802 WP_003410651.1 NZ_CP069077:2342194-2342427 [Mycobacterium tuberculosis]
MSTSTTIRVSTQTRDRLAAQARERGISMSALLTELAAQAERQAIFRAEREASHAETTTQAVRDEDREWEGTVGDGLG

Nucleotide

• Length: 234 bp

>AT189802 NZ_CP091016:1136705-1136953 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A7U4BV50