Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mbcAT/RES-TIGR02293
Location 2249104..2250002 Replicon chromosome
Accession NZ_CP069077
Organism Mycobacterium tuberculosis strain N0072

Toxin (Protein)


Gene name mbcT Uniprot ID P64908
Locus tag JN135_RS10545 Protein ID WP_003410001.1
Coordinates 2249104..2249664 (-) Length 187 a.a.

Antitoxin (Protein)


Gene name mbcA Uniprot ID P64910
Locus tag JN135_RS10550 Protein ID WP_003410003.1
Coordinates 2249661..2250002 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN135_RS10515 2244273..2244926 - 654 WP_003409976.1 cutinase Cfp21 -
JN135_RS10520 2245041..2245271 + 231 WP_003409980.1 DUF167 domain-containing protein -
JN135_RS10525 2245356..2246267 - 912 WP_003409986.1 ArgP/LysG family DNA-binding transcriptional regulator -
JN135_RS10530 2246376..2246975 + 600 WP_003409989.1 L-lysine exporter -
JN135_RS10535 2247391..2247819 + 429 WP_003409992.1 cellulose-binding protein -
JN135_RS10540 2248045..2248584 + 540 WP_003900446.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37) -
JN135_RS10545 2249104..2249664 - 561 WP_003410001.1 RES family NAD+ phosphorylase Toxin
JN135_RS10550 2249661..2250002 - 342 WP_003410003.1 DUF2384 domain-containing protein Antitoxin
JN135_RS10555 2250088..2250345 + 258 WP_003410006.1 hypothetical protein -
JN135_RS10560 2250246..2250581 - 336 WP_003410009.1 dehydrogenase -
JN135_RS10565 2250670..2251014 - 345 WP_003410010.1 type II toxin-antitoxin system toxin endoribonuclease MazF6 -
JN135_RS10570 2251008..2251256 - 249 WP_003410014.1 ribbon-helix-helix protein, CopG family -
JN135_RS10575 2251356..2253671 - 2316 WP_003899120.1 cation transporter ATPase CptG -
JN135_RS10580 2253668..2253940 - 273 WP_003903706.1 DUF1490 family protein -
JN135_RS10585 2253993..2254349 - 357 WP_003410018.1 Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(25-148)

Antitoxin

Antitoxin Xre/MbcA/ParS-like, toxin-binding domain

(69-110)


Sequences

Toxin        


Download         Length: 187 a.a.        Molecular weight: 20248.70 Da        Isoelectric Point: 4.5091

>T189798 WP_003410001.1 NZ_CP069077:c2249664-2249104 [Mycobacterium tuberculosis]
VSDALDEGLVQRIDARGTIEWSETCYRYTGAHRDALSGEGARRFGGRWNPPLLFPAIYLADSAQACMVEVERAAQAASTT
AEKMLEAAYRLHTIDVTDLAVLDLTTPQAREAVGLENDDIYGDDWSGCQAVGHAAWFLHMQGVLVPAAGGVGLVVTAYEQ
RTRPGQLQLRQSVDLTPALYQELRAT

Download         Length: 561 bp

>T189798 NZ_CP091016:c610642-610178 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 12488.33 Da        Isoelectric Point: 4.8359

>AT189798 WP_003410003.1 NZ_CP069077:c2250002-2249661 [Mycobacterium tuberculosis]
MGVNVLASTVSGAIERLGLTYEEVGDIVDASPRSVARWTAGQVVPQRLNKQRLIELAYVADALAEVLPRDQANVWMFSPN
RLLEHRKPADLVRDGEYQRVLALIDAMAEGVFV

Download         Length: 342 bp

>AT189798 NZ_CP091016:c610977-610642 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FKG

Antitoxin

Source ID Structure
PDB 6FKG
AlphaFold DB A0A7U4FAE4

References