Detailed information of TA system TA189794

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2206513..2207216
• Replicon: chromosome
• Accession: NZ_CP069077
• Organism: Mycobacterium tuberculosis strain N0072

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: JN135_RS10260
• Protein ID: WP_003409778.1
• Coordinates: 2206513..2206842 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: JN135_RS10265
• Protein ID: WP_003409780.1
• Coordinates: 2206839..2207216 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN135_RS10240	2202896..2203966	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
JN135_RS10245	2203963..2204478	+	516	WP_003409718.1	flavin reductase family protein	-
JN135_RS10250	2204475..2205536	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
JN135_RS10255	2205533..2206303	+	771	WP_003409775.1	SDR family oxidoreductase	-
JN135_RS10260	2206513..2206842	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JN135_RS10265	2206839..2207216	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
JN135_RS10270	2207213..2207803	-	591	WP_003409784.1	SEC-C metal-binding domain-containing protein	-
JN135_RS10275	2207858..2209222	+	1365	WP_003903691.1	HNH endonuclease signature motif containing protein	-
JN135_RS10280	2209377..2209829	-	453	WP_003899095.1	lipoprotein	-
JN135_RS10285	2209893..2210294	+	402	WP_003409869.1	hypothetical protein	-
JN135_RS10290	2210287..2210469	-	183	WP_003409870.1	hypothetical protein	-
JN135_RS10295	2210583..2210933	-	351	WP_003409871.1	hypothetical protein	-
JN135_RS10300	2210944..2211846	-	903	WP_003409874.1	hypothetical protein	-
JN135_RS10305	2211867..2212058	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T189794 WP_003409778.1 NZ_CP069077:c2206842-2206513 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T189794 NZ_CP091013:2493579-2493917 [Burkholderia cenocepacia]
ATGGTTGACATCCTGCAATCCGTGCCCTACAGTGCGCCTACGTTCAGCATCCGGACCACCGACGTCTTCGAAGCGTGGTT
CGCCGGCCTGCCGGATCGCGTCGCGCGGCGGCGCATCCAGGCGCGCATCGACCGTCTGTCGATGGGCAATCCGGGCGACT
GGAAATCCGCCGGCTCCCCCGTCGTCGAAATGCGTATCGACCACGGCCCCGGCTATCGCGTCCATTACGTGAGGCGCGGA
ACGATATGGGTCATCCTGCTCTGCGGCGGCGATAAATCGACGCAACAGGCCGACATCCGCGCCGCGCACGCAATGCTCGC
GCACCTCGACATGGAGTGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT189794 WP_003409780.1 NZ_CP069077:c2207216-2206839 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT189794 NZ_CP091013:2493921-2494220 [Burkholderia cenocepacia]
ATGGACAAGATCAAGACGCGGCCGTGGGATTCGGCCGAACACCTGAAAACCGAAGACGACATGGCCGACTACTTCGACGC
CTGCCTGCAGGAGGCCGGCGACGATCCGGCCTTCATCGCGCATGCGCTCGGCGTGATCGCCCGTGCCCGCGGCATGTCGC
AGGTCGCACGCGATGCGGGCCTGTCGCGCGAAGGGCTGTACAAGGCGCTGTCGCACGACGGCAATCCGAGCTTCGGCACG
ATCCTGAAGGTCATCAAGGCGCTCGGCCTGCAATTGCACGGCGCCAAGGCTGCCGCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8