Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 1578770..1579425 Replicon chromosome
Accession NZ_CP069077
Organism Mycobacterium tuberculosis strain N0072

Toxin (Protein)


Gene name vapC Uniprot ID A0A7U4FAR1
Locus tag JN135_RS07450 Protein ID WP_003407268.1
Coordinates 1578770..1579171 (-) Length 134 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TIK2
Locus tag JN135_RS07455 Protein ID WP_003407272.1
Coordinates 1579168..1579425 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN135_RS07435 1574245..1575630 - 1386 WP_003407260.1 cytochrome P450 -
JN135_RS07440 1575708..1576742 + 1035 WP_003407264.1 AraC family transcriptional regulator -
JN135_RS07445 1576788..1578515 - 1728 Protein_1466 PE family protein -
JN135_RS07450 1578770..1579171 - 402 WP_003407268.1 type II toxin-antitoxin system VapC family toxin Toxin
JN135_RS07455 1579168..1579425 - 258 WP_003407272.1 CopG family transcriptional regulator Antitoxin
JN135_RS07460 1579508..1580467 - 960 WP_015631299.1 alpha/beta hydrolase -
JN135_RS07465 1580492..1581454 - 963 WP_003407279.1 alpha/beta hydrolase -
JN135_RS07470 1581453..1582190 + 738 WP_031647000.1 lysoplasmalogenase -
JN135_RS07475 1582271..1584238 + 1968 WP_003407285.1 primosomal protein N' -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-108)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-39)


Sequences

Toxin        


Download         Length: 134 a.a.        Molecular weight: 14894.39 Da        Isoelectric Point: 11.6897

>T189789 WP_003407268.1 NZ_CP069077:c1579171-1578770 [Mycobacterium tuberculosis]
MILVDSDVLIAHLRGVVAARDWLVSARKDGPLAISVVSTAELIGGMRTAERREVWRLLASFRVQPATEVIARRAGDMMRR
YRRSHNRIGLGDYLIAATADVQGLQLATLNVWHFPMFEQLKPPFAVPGHRPRA

Download         Length: 402 bp

>T189789 NZ_CP091013:c1076384-1076118 [Burkholderia cenocepacia]
ATGAGCGTACGTCGCGTGCTCTTTACTTCCGACGCATGGGATGACTATGTCTACTGGCAAGGACAGGATCGCAAGACGCT
CAAGCGCATCAACCAGCTCATTCGCGAAACCCAGCGCACGCCGTTCGAAGGCATCGGCAAGCCGGAGCCGTTGAAAGCCA
ATCTCTCCGGCTTCTGGTCGCGCCGTATCGACGATACGAACCGCCTGGTCTACGAGGCTGACGACCTTCAAATCAGCATC
GTGTCCTGCCGCTACCATTACGAGTAG

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9443.58 Da        Isoelectric Point: 6.4631

>AT189789 WP_003407272.1 NZ_CP069077:c1579425-1579168 [Mycobacterium tuberculosis]
MKRTNIYLDEEQTASLDKLAAQEGVSRAELIRLLLNRALTTAGDDLASDLQAINDSFGTLRHLDPPVRRSGGREQHLAQV
WRATS

Download         Length: 258 bp

>AT189789 NZ_CP091013:c1076641-1076381 [Burkholderia cenocepacia]
ATGCGCACGATTCATTTTTCCGATGCCCGCGGCAATCTGAAGACCGTCATCGACCAGGTCGTCGACGATGCGGACGTGAC
GCTCATTACGCGCCGCGATGCACCGAACGCCGTCATCATGTCGCAGGACTATTACGACAGCCTGATGGAGACGGTTCACC
TGCTGCGCTCGCCAGCCAACGTCGCTCATCTCGAACGTTCGATCGCGCAACTGCGCAAGGGCAAAGCGAAAGAACACAAG
CTCGCCGAGGACGATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FAR1

Antitoxin

Source ID Structure
AlphaFold DB G0TIK2

References