Detailed information of TA system TA189786

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1385975..1386663
• Replicon: chromosome
• Accession: NZ_CP069077
• Organism: Mycobacterium tuberculosis strain N0072

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0THR7
• Locus tag: JN135_RS06610
• Protein ID: WP_003406304.1
• Coordinates: 1386232..1386663 (+)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0THR6
• Locus tag: JN135_RS06605
• Protein ID: WP_003406302.1
• Coordinates: 1385975..1386235 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN135_RS06585	1381552..1382376	+	825	WP_003900298.1	trehalose ABC transporter permease SugB	-
JN135_RS06590	1382381..1383562	+	1182	WP_003406299.1	trehalose ABC transporter ATP-binding protein SugC	-
JN135_RS06595	1383639..1384739	-	1101	WP_003406300.1	magnesium/cobalt transporter CorA	-
JN135_RS06600	1384910..1385899	+	990	WP_003406301.1	malate dehydrogenase	-
JN135_RS06605	1385975..1386235	+	261	WP_003406302.1	type II toxin-antitoxin system antitoxin VapB33	Antitoxin
JN135_RS06610	1386232..1386663	+	432	WP_003406304.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN135_RS06615	1386686..1388372	-	1687	Protein_1307	PE family protein	-
JN135_RS06620	1388552..1389412	+	861	WP_003903377.1	glycine betaine ABC transporter substrate-binding protein	-
JN135_RS06625	1389493..1390323	-	831	WP_003898788.1	SDR family NAD(P)-dependent oxidoreductase	-
JN135_RS06630	1390380..1390673	-	294	WP_003898789.1	type II toxin-antitoxin system mRNA interferase RelE	-
JN135_RS06635	1390670..1390939	-	270	WP_003406322.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (2-142)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 15873.08 Da
• Isoelectric Point: 5.8838

>T189786 WP_003406304.1 NZ_CP069077:1386232-1386663 [Mycobacterium tuberculosis]
VIIPDINLLLYAVITGFPQHRRAHAWWQDTVNGHTRIGLTYPALFGFLRIATSARVLAAPLPTADAIAYVREWLSQPNVD
LLTAGPRHLDIALGLLDKLGTASHLTTDVQLAAYGIEYDAEIHSSDTDFARFADLKWTDPLRE

Nucleotide

• Length: 432 bp

>T189786 NZ_CP091012:c801587-801264 [Burkholderia cenocepacia]
ATGAATCGAGGGAGCATTGTCACAGTCGCGCTCCAAGGTGATTTCGGCAAGGCTCGGCCGGCGCTCGTGGTGCAGTCGGA
CCTGTTTGCCGAACATCCCAGTGTAACGTTGCTGCTAATGTCGAGCTCAATGGTCGATGCGCCCTTGATACGCATTACTG
TTGTCCCATCCGAGCAGAACGGCTTGCGCATGCCGTCCCAGATTGCCGTAGACAAAATGTTCACCGTGCGCCGCGAGAAA
ATCGGTCGCCCCATCGGCCACCTCGAAGATGAACTGATGGTTGCCGTCAATCGTTCGCTACTCGTTTTTCTTGGGCTAGC
GTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9918.25 Da
• Isoelectric Point: 6.4755

>AT189786 WP_003406302.1 NZ_CP069077:1385975-1386235 [Mycobacterium tuberculosis]
MRTTLTLDDDVVRLVEDAVHRERRPMKQVINDALRRALAPPVKRQEQYRLEPHESAVRSGLDLAGFNKLADELEDEALLD
ATRRAR

Nucleotide

• Length: 261 bp

>AT189786 NZ_CP091012:c801796-801584 [Burkholderia cenocepacia]
ATGGCAGCTACCACTTCGGAGCGTGTTCGAATTCACCGCGAGAATCTTCGTGCGGCCGGGCTACGCCCAATTCAGATATG
GGTGCCCGACGTCCGCCGGCCGGGTTTCGCCGATGAATGCGCTCGGCAATCGCGCATGGTCCACCAGAGCATCGACGAGA
ACGATCTGCTCGATTTCATTGAACAGGCTACGGACCTCGGACACTCACAATGA

Structures

Toxin

• AlphaFold DB: G0THR7

Antitoxin

• AlphaFold DB: G0THR6