Detailed information of TA system TA189785

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1241110..1241678
• Replicon: chromosome
• Accession: NZ_CP069077
• Organism: Mycobacterium tuberculosis strain N0072

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: JN135_RS05940
• Protein ID: WP_003405865.1
• Coordinates: 1241304..1241678 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: JN135_RS05935
• Protein ID: WP_003405863.1
• Coordinates: 1241110..1241307 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN135_RS05915	1237151..1237789	-	639	WP_003898730.1	lipid droplet-associated protein	-
JN135_RS05920	1237879..1238886	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
JN135_RS05925	1238903..1239886	-	984	WP_003903326.1	hypothetical protein	-
JN135_RS05930	1239949..1241022	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
JN135_RS05935	1241110..1241307	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JN135_RS05940	1241304..1241678	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN135_RS05945	1241881..1242579	+	699	WP_003898733.1	hypothetical protein	-
JN135_RS05950	1242697..1242882	+	186	WP_003901093.1	hypothetical protein	-
JN135_RS05955	1242809..1243084	-	276	WP_003405867.1	hypothetical protein	-
JN135_RS05960	1243327..1243650	+	324	WP_003405871.1	putative quinol monooxygenase	-
JN135_RS05965	1243665..1244363	-	699	WP_031646953.1	hypothetical protein	-
JN135_RS05970	1244397..1244606	+	210	WP_003911400.1	hypothetical protein	-
JN135_RS05975	1244558..1245328	-	771	Protein_1178	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T189785 WP_003405865.1 NZ_CP069077:1241304-1241678 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T189785 NZ_CP091007:40937-41203 [Staphylococcus epidermidis]
ATGAATAAGTACAATATAACTTTCACACCTACAGCTTTTGAAGATTATAAGTATTGGCAAACACAAGATAAAAAACTATT
AAAGAAAATTAATAGTTTGATTAAGAGTATTCAAAGAGATGGCATTTTAGAGGGTGAAGGCAAGGCCGAGCCTTTGAAAG
GTAATTTGAAAGGTTACTATAGTAGAAGAATAAATCACGAGCATAGATTAGTTTATACTATTAAAGACCCTAGTATTATA
ATCATTGCATGTAGATATCATTATTAA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT189785 WP_003405863.1 NZ_CP069077:1241110-1241307 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT189785 NZ_CP091007:40690-40944 [Staphylococcus epidermidis]
ATGACTGTTTTAACTTATTCAAACGCACGTAAGGATTTCCGAAAACTAATAGATAAAGTAAATGATGATAGTGATACAGT
AACTATTACAACTAATGACCGTAACGCTGTATTGATTAGTGAAGATGATTATAACTCAATTATGGAAACTCTCTACTTAC
AACAATCACCAGTCAATGCTAAACGCTTAGCTCAATCAATGGAAGAAGCTGAAAGAGTCAATACTGTAGAGGTAGATATA
ACAACTTATGAATAA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07