Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1232354..1232985 Replicon chromosome
Accession NZ_CP069077
Organism Mycobacterium tuberculosis strain N0072

Toxin (Protein)


Gene name mazF Uniprot ID A0A7U4F9D0
Locus tag JN135_RS05885 Protein ID WP_003405820.1
Coordinates 1232354..1232665 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TGZ7
Locus tag JN135_RS05890 Protein ID WP_003405836.1
Coordinates 1232665..1232985 (-) Length 107 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN135_RS05865 1227835..1229259 - 1425 WP_003405805.1 class II fumarate hydratase -
JN135_RS05870 1229290..1230378 - 1089 WP_017351234.1 class II fructose-bisphosphatase -
JN135_RS05875 1230434..1231078 + 645 WP_003915494.1 DUF4245 domain-containing protein -
JN135_RS05880 1231085..1232242 - 1158 WP_003898727.1 AI-2E family transporter -
JN135_RS05885 1232354..1232665 - 312 WP_003405820.1 type II toxin-antitoxin system toxin endoribonuclease MazF3 Toxin
JN135_RS05890 1232665..1232985 - 321 WP_003405836.1 type II toxin-antitoxin system antitoxin MazE3 Antitoxin
JN135_RS05895 1232995..1234531 + 1537 Protein_1162 carboxylesterase/lipase family protein -
JN135_RS05900 1234538..1235650 - 1113 WP_003405840.1 3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase -
JN135_RS05905 1235660..1235917 - 258 WP_003405844.1 exodeoxyribonuclease VII small subunit -
JN135_RS05910 1235907..1237154 - 1248 WP_003405846.1 exodeoxyribonuclease VII large subunit -
JN135_RS05915 1237151..1237789 - 639 WP_003898730.1 lipid droplet-associated protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-85)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 11061.82 Da        Isoelectric Point: 4.9371

>T189784 WP_003405820.1 NZ_CP069077:c1232665-1232354 [Mycobacterium tuberculosis]
MRPIHIAQLDKARPVLILTREVVRPHLTNVTVAPITTTVRGLATEVPVDAVNGLNQPSVVSCDNIQTIPVCDLGRQIGYL
LASQEPALAEAIGNAFDLDWVVA

Download         Length: 312 bp

>T189784 NZ_CP091007:c1897-1631 [Staphylococcus epidermidis]
ATGGCTAAATATAAAATCAACATTAAGAATTCAGCGAAGACAGATTTAAAGAAGATAAAACGTTCTCACTTACAAGAACA
ATTTTTGAAAATCGTAGAAATACTAAAATATAATCCATATCAACAAAGTCAATCCTTTGAAAAATTGCACCCTAAACATT
TAGGACGATACTCAAGACGTATCAATCATCAACATAGAGTTGTCTATACAATTGATGAGGAAAATAAAGAAGTATTAATA
TTAGCAACTTGGTCTCATTATGAATAA

Antitoxin


Download         Length: 107 a.a.        Molecular weight: 11394.83 Da        Isoelectric Point: 5.1236

>AT189784 WP_003405836.1 NZ_CP069077:c1232985-1232665 [Mycobacterium tuberculosis]
MYLPWGVVLAGGANGFGAGAYQTGTICEVSTQIAVRLPDEIVAFIDDEVRGQHARSRAAVVLRALERERRRRLAERDAEI
LATNTSATGDLDTLAGHCARTALDID

Download         Length: 321 bp

>AT189784 NZ_CP091007:c2154-1897 [Staphylococcus epidermidis]
ATGAAAAGTAAAACACCAACAGAAGCTCGTAAAGATTTTTATCAGTTATTAAAACGTGTGAATAATGACCATGAACCAGT
ATATATTGAAGGCAAATATGATGAGAGTAATGCTGTTATTATAGGCATGGAAGATTGGCGTAGCATTAATGAAACGATAT
ATCTTGAGGCTACAGGCACAATGGACAAAGTTAGAGAACGTGAGCATGACAACAGTGGTTATACAAACATAGATGATATT
GATTGGGCTAGACTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4F9D0

Antitoxin

Source ID Structure
AlphaFold DB G0TGZ7

References