Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapC-mazE/PIN(toxin)
Location 759035..759827 Replicon chromosome
Accession NZ_CP069077
Organism Mycobacterium tuberculosis strain N0072

Toxin (Protein)


Gene name vapC Uniprot ID P9WFB2
Locus tag JN135_RS03490 Protein ID WP_003403386.1
Coordinates 759390..759827 (-) Length 146 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TQE0
Locus tag JN135_RS03485 Protein ID WP_003403381.1
Coordinates 759035..759280 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN135_RS03455 754055..755560 + 1506 WP_003403360.1 carotenoid cleavage oxygenase -
JN135_RS03460 755641..756651 + 1011 WP_031653722.1 ABC transporter ATP-binding protein -
JN135_RS03465 757039..757422 - 384 WP_003403365.1 ribonuclease VapC6 -
JN135_RS03470 757517..757672 - 156 WP_003403368.1 type II toxin-antitoxin system VapB family antitoxin -
JN135_RS03475 757748..758464 - 717 WP_003903122.1 CPBP family intramembrane metalloprotease -
JN135_RS03480 758740..759048 - 309 WP_003403376.1 type II toxin-antitoxin system PemK/MazF family toxin -
JN135_RS03485 759035..759280 - 246 WP_003403381.1 ribbon-helix-helix protein, CopG family Antitoxin
JN135_RS03490 759390..759827 - 438 WP_003403386.1 type II toxin-antitoxin system VapC family toxin Toxin
JN135_RS03495 759824..760078 - 255 WP_003911263.1 antitoxin VapB7 -
JN135_RS03500 760192..762555 + 2364 WP_003403397.1 arylsulfatase AtsD -
JN135_RS03505 762618..762944 + 327 WP_003403401.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
JN135_RS03510 762856..763194 + 339 WP_003403405.1 PIN domain-containing protein -
JN135_RS03515 763191..763364 + 174 WP_003898549.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-126)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 146 a.a.        Molecular weight: 15245.39 Da        Isoelectric Point: 5.1865

>T189778 WP_003403386.1 NZ_CP069077:c759827-759390 [Mycobacterium tuberculosis]
MIVLDTTVLVYAKGAEHPLRDPCRDLVAAIADERIAATTTAEVIQEFVHVRARRRDRSDAAALGRVTMPNCSRRYSPSIE
ATSKRGLTLFETTPGLEACDAVLAAVAASAGATALVSADPAFADLSDVVHVIPDAAGMVSLLGDR

Download         Length: 438 bp

>T189778 NZ_CP090998:1909175-1909363 [Staphylococcus epidermidis]
ATGAGCTTACATTTCCAAATTTTGCTGTGGCTTTCTATCTTATTTATCATCGCAGGAACGATATTATTGGTAACAATGCT
TAAAACTAAAAAAGAAGAACGAAAAGAATCTTATCTAGGCTTTACTGTAATTTTCTTAATCTTTGGTTTTGCCATCTTAA
TTTATACTTTTATATTCGGAATTTTATAA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9169.01 Da        Isoelectric Point: 4.2070

>AT189778 WP_003403381.1 NZ_CP069077:c759280-759035 [Mycobacterium tuberculosis]
MLSFRADDHDVDLADAWARRLHIGRSELLRDALRRHLAALAADQDVQAYTERPLTDDENALAEIADWGPAEDWADWADAA
R

Download         Length: 246 bp

>AT189778 NZ_CP090998:1908893-1909150 [Staphylococcus epidermidis]
ATGAAATTAAACAAAGCAAATATATTCAATTTGATATTCACCATTTTATTTTTTTCATTTAACATTCTCATAACCTATAA
CGCAAATATAGATTATAAGTTATGGTTAATACCTGGTTTAGCAATTTGTGGTTTTGCTCTTTTTTCAAGCTTGACCTTAG
TAATTATCTATAGTGATTTATTCAGTGAAATATTATTTTTTATCAACATTATCTTAGCACTGTATTACATTTATCCGATT
TTTTATGAATTTGTCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BSW5

Antitoxin

Source ID Structure
AlphaFold DB G0TQE0

References