Detailed information of TA system TA189771

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/AbrB(antitoxin)
• Location: 700681..701327
• Replicon: chromosome
• Accession: NZ_CP069077
• Organism: Mycobacterium tuberculosis strain N0072

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ88
• Locus tag: JN135_RS03140
• Protein ID: WP_003403137.1
• Coordinates: 700681..701094 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ89
• Locus tag: JN135_RS03145
• Protein ID: WP_003403139.1
• Coordinates: 701091..701327 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN135_RS03120	696764..698314	+	1551	WP_003403119.1	MlaD family protein	-
JN135_RS03125	698366..698758	-	393	WP_003403122.1	type II toxin-antitoxin system toxin ribonuclease C4	-
JN135_RS03130	698755..699012	-	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	-
JN135_RS03135	699195..700430	-	1236	WP_003403128.1	ATP-binding protein	-
JN135_RS03140	700681..701094	-	414	WP_003403137.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN135_RS03145	701091..701327	-	237	WP_003403139.1	type II toxin-antitoxin system antitoxin VapB27	Antitoxin
JN135_RS03150	701431..701937	-	507	WP_003900973.1	two component system sensor kinase HK1	-
JN135_RS03155	702051..702581	-	531	WP_003917320.1	two component system sensor kinase HK2	-
JN135_RS03160	702565..703260	-	696	WP_200861092.1	two-component system response regulator TcrA	-
JN135_RS03165	703383..703694	+	312	WP_003403164.1	hypothetical protein	-
JN135_RS03170	703766..704716	+	951	WP_003907436.1	DUF1259 domain-containing protein	-
JN135_RS03175	704957..705541	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
JN135_RS03180	705543..706253	+	711	Protein_631	IS607 family element RNA-guided endonuclease TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-126)

Antitoxin

• SpoVT-AbrB domain (3-43)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14786.14 Da
• Isoelectric Point: 8.1405

>T189771 WP_003403137.1 NZ_CP069077:c701094-700681 [Mycobacterium tuberculosis]
VKPPLAVDTSVAIPLLVRTHTAHAAVVAWWAHREAALCGHALAETYSVLTRLPRDLRLAPMDAARLLTERFAAPLLLSSR
TTEHLPRVLAQFEITGGAVYDALVALAAAEHRAELATRDARAKDTYEKIGVHVVVAA

Nucleotide

• Length: 414 bp

>T189771 NZ_CP090993:963733-963912 [Staphylococcus epidermidis]
ATGGCGAACAAAAAAGATTCCAAATTAAATTATCATGAAGAAGAAAATGCTATGGTTACCGACTTAGATGATTTAAAAGA
ATTAGGAAAAGAAATGGAACAGATTTCACAGGAAAATGATGAAGAAAAATTAAATCAATCTCATGATAACGAAGTTCGTT
CAGATTTAAAAAAACAGTAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8212.41 Da
• Isoelectric Point: 5.0351

>AT189771 WP_003403139.1 NZ_CP069077:c701327-701091 [Mycobacterium tuberculosis]
MKAVVDAAGRIVVPKPLREALGLQPGSTVEISRYGAGLHLIPTGRTARLEEENGVLVATGETTIDDEVVFGLIDSGRK

Nucleotide

• Length: 237 bp

>AT189771 NZ_CP090993:963110-963709 [Staphylococcus epidermidis]
ATGGCAATGAATTTTAAAGTATTTGATAATAGTGAAAAAGTAGCAGAATATACTGCAGATATTCTACGTAAGCAATTTAA
TAATAATCCAACTACTATTGCAGGATTTCATTTGAGCAAAGAACATGCACCAGTGTTTGATGAACTTAAGAAAAATGTAG
AAAATCATACAGTTGATTTCAGTCAAATAAATATTTTAGATTATGATGATAATCATTCTTATTATGAGGCTTTGGGTGTG
CCAACAGGCCAAATTTATTCCATTTCTTACGAAAATGATGCAATTGACTTTATCTCAGATAAGATTAAGACTAAAGAAAA
TAAAGGCAAACTGACAATGCAAGTCCTTACGATTGATGAAAATGGTAAACTTGACATTAGCGTTCGTCAAGGTTTGATGG
AAGCTAGAGAAATTTTCTTAGTTATTACTGGTGCTAATAAACGCGATATGGTAGAAAAACTTTACCGTGAAAATGGTAAG
ACTAGTTTTGAACCATCGGATTTAAAAGCACACCGTATGGTCAATGTTATCTTAGATAAAGAAGCGGCTGCAGGCTTATC
TGAAGACGTAAAAGAGTACTTCACTGCACGTTTTGCATAA

Structures

Toxin

• AlphaFold DB: G0TQ88

Antitoxin

• AlphaFold DB: G0TQ89